The NO donor sodium nitroprusside: evaluation of skeletal muscle vascular and metabolic dysfunction.
The NO donor sodium nitroprusside: evaluation of skeletal muscle vascular and metabolic dysfunction.
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DOI：
10.1016/j.mvr.2012.11.006
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发表时间：
2013-01
影响因子：
3.1
通讯作者：
Poole, David C.
中科院分区：
文献类型：
--
作者：
Hirai, Daniel M.;Copp, Steven W.;Ferguson, Scott K.;Holdsworth, Clark T.;Musch, Timothy I.;Poole, David C.
一氧化氮(NO)供体硝普钠(SNP)可促进氰化物诱导的毒性和接近最大血管扩张的全身和/或局部反应。这些假说被检验为：1)静息和收缩血流量、氧利用(V̇O2)和微血管O2压(PO2 Mv)的残余损害；2)静息PO2 mv的显著降压和升高。按顺序执行两种灌流方案：1)Krebs-Henseleit(对照1)、SNP(300µM，灌流研究中常用的剂量)和Krebs-Henseleit(对照2)；2)随机顺序300和1200µM SNP。测定静息状态和电刺激(1 Hz)收缩时的斜方肌血流量(放射性标记微球)、V̇O2(Fick计算)和PO2 mv(磷光猝灭)。SNP前后的斜方肌血流量、静息和收缩时的V̇O2或PO2 mv无差异(对照1和对照2均为P>；0.0 5)。在剂量方面，硝普钠使静息PO2 mv逐渐升高(p<；0.05)，而平均动脉压仅在较高浓度时降低(p<；0.05)。与我们的假设相反，骨骼肌灌流与NO供体SNP(300微米)改善了从静息到收缩转变(PO2 mv动力学)的微血管氧合，而不会导致肌肉血流动力学或代谢控制的残余损害或损害全身血流动力学。这些数据表明，SNP灌流(300微米)是一种有效和重要的工具，用于评估NO在静息和收缩骨骼肌功能中的功能作用，而不会引起与氰化物积聚和中毒一致的残余改变。
The nitric oxide (NO) donor sodium nitroprusside (SNP) may promote cyanide-induced toxicity and systemic and/or local responses approaching maximal vasodilation. The hypotheses were tested that SNP superfusion of the rat spinotrapezius muscle exerts 1) residual impairments in resting and contracting blood flow, oxygen utilization (V̇O2) and microvascular O2 pressure (PO2mv); and 2) marked hypotension and elevation in resting PO2mv. Two superfusion protocols were performed: 1) Krebs-Henseleit (control 1), SNP (300 µM; a dose used commonly in superfusion studies) and Krebs-Henseleit (control 2), in this order; 2) 300 and 1200 µM SNP in random order. Spinotrapezius muscle blood flow (radiolabeled microspheres), V̇O2 (Fick calculation) and PO2mv (phosphorescence quenching) were determined at rest and during electrically-induced (1 Hz) contractions. There were no differences in spinotrapezius blood flow, V̇O2 or PO2mv at rest and during contractions pre- and post-SNP condition (control 1 and control 2; p>0.05 for all). With regard to dosing, SNP produced a graded elevation in resting PO2mv (p<0.05) with a reduction in mean arterial pressure only at the higher concentration (p<0.05). Contrary to our hypothesis, skeletal muscle superfusion with the NO donor SNP (300 µM) improved microvascular oxygenation during the transition from rest to contractions (PO2mv kinetics) without precipitating residual impairment of muscle hemodynamic or metabolic control or compromising systemic hemodynamics. These data suggest that SNP superfusion (300 µM) constitutes a valid and important tool for assessing the functional roles of NO in resting and contracting skeletal muscle function without incurring residual alterations consistent with cyanide accumulation and poisoning.
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影响因子：
2.3
作者：
Kindig, CA;McDonough, P;Poole, DC
通讯作者：
Poole, DC
影响因子：
3.3
作者：
Hirai, Daniel M.;Copp, Steven W.;Poole, David C.
通讯作者：
Poole, David C.
影响因子：
3.3
作者：
Behnke, BJ;Padilla, DJ;Poole, DC
通讯作者：
Poole, DC
影响因子：
6.3
作者：
Hirai, D. M.;Copp, S. W.;Poole, D. C.
通讯作者：
Poole, D. C.
影响因子：
2.3
作者：
Hirai, Daniel M.;Copp, Steven W.;Musch, Timothy I.
通讯作者：
Musch, Timothy I.