MicroRNA Profile Differentiates Head and Neck Keloid and Adjacent Normal Skin Tissue.
MicroRNA Profile Differentiates Head and Neck Keloid and Adjacent Normal Skin Tissue.
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MicroRNA 谱可区分头颈部瘢痕疙瘩和邻近的正常皮肤组织。
DOI：
10.1089/fpsam.2020.0414
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发表时间：
2021
影响因子：
2
通讯作者：
Mi,Qing-Sheng
中科院分区：
文献类型：
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作者：
Jones,LamontR;Levin,AlbertM;Dai,Xiangguo;Datta,Indrani;Li,Jia;Yin,Congcong;Mi,Qing-Sheng
由于缺乏瘢痕疙瘩特异性生物标志物，瘢痕疙瘩的研究和治疗受到阻碍。匹配的正常皮肤是消除混杂的重要对照。然而，尽管在临床上是正常的和可接近的，但由于瘢痕疙瘩发育的风险或邻近组织可能含有瘢痕疙瘩组织，人们对使用远距离供体部位表示担忧。一个区分瘢痕疙瘩和邻近正常组织的生物标记物将解决这些问题，并提供一个分子标准，提高瘢痕疙瘩研究研究的可比性。最近，为了更好地理解瘢痕疙瘩的发病机制，人们已经转向对表观遗传修饰的更深层次的描述。1由于其特异性和调节基因表达的能力，microRNAs(MiRNAs)是一类表观遗传修饰物，可以作为瘢痕疙瘩的功能性分子生物标志物。因此，我们试图识别能够区分瘢痕疙瘩和邻近正常组织的miRNA。通过批准的IRB方案获得新鲜的、原发的和未经处理的头颈部瘢痕疙瘩和邻近匹配的正常皮肤。临床确定邻近正常皮肤。发现和验证队列分别由15名和7名患者组成(表1)。用Ambion RiboPure‘Kit提取总RNA。分析752个miRNA的TaqMan miRNA阵列(卡A和B)用于根据负Delta周期阈值来确定miRNA的表达。全局平均归一化
Keloid research and treatment are hindered by the lack of keloid-specific biomarkers. Matched normal skin is an important control to eliminate confounding. However, although clinically normal and accessible, there are concerns about the use of either distant donor sites because of the risk of keloid development or the adjacent tissue, as it may harbor keloid tissue. A biomarker that distinguishes keloid and adjacent normal tissue would address these concerns and provide a molecular standard that would improve comparability across keloid research studies. Recently, there has been a shift toward a deeper characterization of epigenetic modifications to better understand keloid pathogenesis. 1 Because of their specificity and ability to modulate gene expression, microRNAs (miRNAs) are one such class of epigenetic modifiers that could be utilized as a functional molecular biomarker of keloids given that miRNAs are known to influence keloids2. Therefore, we sought to identify miRNA able to differentiate keloid and adjacent normal tissue.Fresh, primary, and untreated head and neck (H&N) keloid and adjacent matched normal skin were obtained through an approved IRB protocol. Adjacent normal skin was determined clinically. Discovery and validation cohorts were composed of 15 and 7 patients, respectively (Table 1). Total RNA was extracted, using the Ambion RiboPureÔ kit. The TaqMan Ò MiRNA array (card A & B), which profiles 752 miRNAs, was used to determine miRNA expression in terms of negative Delta Cycle Threshold values. Global mean normalization