Stimuli-responsive functionalized mesoporous silica nanoparticles for drug release in response to various biological stimuli
Stimuli-responsive functionalized mesoporous silica nanoparticles for drug release in response to various biological stimuli
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DOI：
10.1039/c3bm60148j
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发表时间：
2014-01-01
影响因子：
6.6
通讯作者：
Gooding, J. Justin
中科院分区：
文献类型：
--
作者：
Chen, Xin;Cheng, Xiaoyu;Gooding, J. Justin
合成了一种硅基介孔纳米球（MSN）控释给药系统。该系统使用L-半胱氨酸衍生的金纳米颗粒（AuNP），使用Cu 2+作为桥接离子与MSN结合。AuNP充当可移除的帽，其阻碍药物分子在氨基官能化的MSN介孔框架内的释放。如使用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四唑（MTT）测定所揭示的，修饰的MSN本身对活细胞表现出可忽略的细胞毒性。药物递送系统需要两种生物刺激之一来触发药物释放。这些刺激是：低pH（pH < 5）;或升高的三磷酸腺苷（ATP）水平（浓度> 4 mM）。生物控制释放的可行性通过刺激诱导除去MSN上的AuNP帽来证明，从而释放抗癌药物多柔比星。我们设想，这种MSN系统可以在开发新一代控释运载工具方面发挥重要作用。
A silica-based mesoporous nanosphere (MSN) controlled-release drug delivery system has been synthesized and characterized. The system uses L-cysteine derivatized gold nanoparticles (AuNPs), bound to the MSNs using Cu2+ as a bridging ion. The AuNPs serve as removable caps that hinder the release of drug molecules inside the amino functionalized MSN mesoporous framework. The modified MSNs themselves exhibit negligible cytotoxicity to living cells, as revealed using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay. The drug delivery system requires one of two biological stimuli to trigger drug release. These stimuli are either: low pH (pH < 5); or elevated levels of adenosine triphosphate (ATP) (concentration > 4 mM). The feasibility of biologically controlled release was demonstrated through the stimuli-induced removal of the AuNP caps over the MSN releasing the anticancer drug doxorubicin. We envisage that this MSN system could play a significant role in developing new generations of controlled-release delivery vehicles.