THE GENETIC ORGANIZATION OF THE MAU GENE-CLUSTER OF THE FACULTATIVE AUTOTROPH PARACOCCUS-DENITRIFICANS
THE GENETIC ORGANIZATION OF THE MAU GENE-CLUSTER OF THE FACULTATIVE AUTOTROPH PARACOCCUS-DENITRIFICANS
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DOI：
10.1016/s0006-291x(05)80007-5
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发表时间：
1992-05-15
影响因子：
3.1
通讯作者：
LIDSTROM, ME
中科院分区：
文献类型：
--
作者：
CHISTOSERDOV, AY;BOYD, J;LIDSTROM, ME
克隆了副球菌的一个基因簇。对携带甲胺脱氢酶小亚基和大亚基基因的克隆片段的区域进行鉴定和测序。鉴定了MADH小亚基基因和MADH大亚基基因的开放阅读框。在该序列中还发现了另外三个编码未知功能多肽的开放阅读框。小亚基基因序列分析结果表明，该MADH小亚基多肽具有一个不寻常的前导序列，并含有色氨酸醌辅因子。MADH小亚基基因及其上游部分开放阅读框存在于M.对AM 1和P.科幻小说有相当大的相似性。序列数据已被用于改进的X-射线晶体结构的MADH从P. acetificans，并已确定关键的保守残基。
Themaugene cluster fromParacoccus denitrificanswas cloned. The regions of a cloned fragment carrying genes for the small and the large subunit of the methylamine dehydrogenase were identified and sequenced. Open reading frames for the MADH small subunit gene and the MADH large subunit gene were identified. Three other open reading frames coding polypeptides with unknown function were found in the sequence. The small subunit gene sequence data reveal that the MADH small subunit polypeptide fromP.denitrificanshas an unusual leader sequence and contains the tryptophan tryptophyl quinone cofactor. The MADH small subunit genes and the parts of the open reading frames found upstream of them in the genome ofM. extorquensAM1 andP. denitrificanshave considerable similarity. The sequence data have been used for refinement of the X-ray crystallographic structure of the MADH fromP.denitrificans, and key conserved residues have been identified.