Three-dimensional structure determination from a single view
Three-dimensional structure determination from a single view
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DOI：
10.1038/nature08705
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发表时间：
2010-01-14
期刊：
影响因子：
64.8
通讯作者：
Miao, Jianwei
中科院分区：
文献类型：
--
作者：
Raines, Kevin S.;Salha, Sara;Miao, Jianwei
确定物质三维结构的能力极大地促进了我们对自然的理解。传统上，用于物体的三维（3D）结构确定的最广泛使用的方案是通过在各种样品取向上获取多个测量值来实现的，如在晶体学和断层摄影术（1，2）的情况下，或者通过扫描样品的一系列薄切片来实现的，如在共焦显微镜（3）中。在这里，我们提出了一种3D成像模式，称为ankylography（来自希腊单词ankylos的意思是'弯曲'和graphein的意思是'书写'），在某些情况下，使完整的3D结构确定从一个单一的曝光使用单色入射光束。我们证明，当一个有限的物体的衍射图案采样在足够细的尺度上的埃瓦尔德球，该对象的3D结构是在原则上由2D球形图案。我们证实了理论分析进行3D数值重建的硅酸钠玻璃结构在2埃的分辨率，和一个单一的脊髓灰质炎病毒在2- 3 nm的分辨率，从二维球面衍射图案单独。使用从软X射线激光衍射数据，我们还提供了一个初步的演示，ankylography是实验上可行的，从一个单一的2D衍射图案获得一个3D图像的测试对象。随着进一步的发展，这种从单一视图获得完整的三维结构信息的方法可以在物理和生命科学中找到广泛的应用。
The ability to determine the structure of matter in three dimensions has profoundly advanced our understanding of nature. Traditionally, the most widely used schemes for three-dimensional (3D) structure determination of an object are implemented by acquiring multiple measurements over various sample orientations, as in the case of crystallography and tomography(1,2), or by scanning a series of thin sections through the sample, as in confocal microscopy(3). Here we present a 3D imaging modality, termed ankylography (derived from the Greek words ankylos meaning 'curved' and graphein meaning 'writing'), which under certain circumstances enables complete 3D structure determination from a single exposure using a monochromatic incident beam. We demonstrate that when the diffraction pattern of a finite object is sampled at a sufficiently fine scale on the Ewald sphere, the 3D structure of the object is in principle determined by the 2D spherical pattern. We confirm the theoretical analysis by performing 3D numerical reconstructions of a sodium silicate glass structure at 2 angstrom resolution, and a single poliovirus at 2-3nm resolution, from 2D spherical diffraction patterns alone. Using diffraction data from a soft X-ray laser, we also provide a preliminary demonstration that ankylography is experimentally feasible by obtaining a 3D image of a test object from a single 2D diffraction pattern. With further development, this approach of obtaining complete 3D structure information from a single view could find broad applications in the physical and life sciences.