NUCLEOTIDE-SEQUENCE AND TRANSCRIPTION START POINT OF THE PHOSPHOGLYCERATE TRANSPORTER GENE OF SALMONELLA-TYPHIMURIUM
NUCLEOTIDE-SEQUENCE AND TRANSCRIPTION START POINT OF THE PHOSPHOGLYCERATE TRANSPORTER GENE OF SALMONELLA-TYPHIMURIUM
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DOI：
10.1128/jb.170.8.3421-3426.1988
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发表时间：
1988-08-01
影响因子：
3.2
通讯作者：
HONG, JS
中科院分区：
文献类型：
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作者：
GOLDRICK, D;YU, GQ;HONG, JS
鉴定了鼠伤寒沙门氏菌磷酸甘油酸转运蛋白基因及其多肽产物，并测定了该基因的核苷酸序列。预测的翻译产物是一种含有406个氨基酸残基的蛋白质，具有极强的疏水性，这与它在膜运输中的作用是一致的。水解分析表明，该蛋白有至少20个氨基酸残基的8个跨膜片段。转录起始点被定位在相对于假定的翻译起始点的-44位置。将PgtP与参与6-磷酸己糖和3-磷酸甘油运输的膜结合蛋白UhpT和GlpT进行比较，发现它们之间的氨基酸序列具有非常高的相似性，这不仅反映了这些多肽之间相似的结构和功能，而且反映了它们具有共同的进化起源。
We identified the phosphoglycerate transporter gene of Salmonella tymphimurium and its polypeptide product and determined the nucleotide sequence of the gene. The predicted translation product was a protein of 406 amino acid residues and was extremely hydrophobic, a feature that is consistent with its role in membrane transport. Hydropaythy analysis suggested that there are eight transmembrane segments of at least 20 amino acid residues for the protein. The transcription start point was mapped to lie at position -44 relative to the putative translational initiation start point. Comparison of PgtP with UhpT and GlpT, the membrane-bound proteins involved in the transport of hexose-6-phosphate and glycerol-3-phosphate, respectively, revealed a very high degree of amino acid sequence similarity among them, reflecting not only similar structures and functions among these polypeptides but also a common evolutionary origin for them.