Isolation and characterization of the Haemophilus influenzae uvrA gene
Isolation and characterization of the Haemophilus influenzae uvrA gene
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DOI：
10.1016/0378-1119(96)00264-8
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发表时间：
1996-10-24
期刊：
影响因子：
3.5
通讯作者：
StGeme, JW
中科院分区：
文献类型：
--
作者：
delaMorena, ML;Hendrixson, DR;StGeme, JW
克隆了流感嗜血杆菌（Haemophilusinfluenzae，Hi）的uvrA基因，并进行了序列测定。推导的氨基酸序列分析表明，81%的同源性和90%的相似性与大肠杆菌UvrA蛋白。与Hi uvrA在DNA修复中的作用一致，Hi uvrA突变体表现出对UV照射的敏感性增加。此外，Hi uvrA能够补充E. coliuvrA位点。
The uvr A gene of Haemophilus influenzae (Hi) was cloned and sequenced. Analysis of the deduced amino acid sequence revealed 81% identity and 90% similarity with the Escherichia coli UvrA protein. Consistent with a role of Hi uvrA in DNA repair, a Hi uvr A mutant exhibited increased sensitivity of UV irradiation. Furthermore, Hi uvrA was able to complement a mutation in the E. coli uvr A locus.