Functional diameters of alveolar microvessels at high lung volume in zone II
Functional diameters of alveolar microvessels at high lung volume in zone II
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DOI：
10.1152/jappl.1998.85.1.47
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发表时间：
1998-07-01
影响因子：
3.3
通讯作者：
Rodenkirch, LA
中科院分区：
文献类型：
--
作者：
Conhaim, RL;Rodenkirch, LA
为了估计肺泡微血管的功能直径，我们分别在充气、肺动脉和左心房压力为25、30和0 cmH（2）O时，用荧光乳胶颗粒（1直径/肺）灌注离体大鼠肺。我们使用共聚焦显微镜计数隔微血管内的乳胶颗粒，并使用流式细胞术计数静脉流出液中的颗粒浓度。我们发现，在45 +/-12%、31 +/-12%和25 +/-9%的受检肺泡中，间隔血管内分别存在直径为1、2和4 μ m的颗粒。5 μ m直径的颗粒不存在于间隔血管中，但存在于一小部分角血管中。静脉流出液中1、2、4和5 μ m直径颗粒的颗粒浓度分别为动脉流入液的54 +/- 28、67 +/- 32、2.2 /- 0.3和0.4 +/-0.3%。静脉流出液中不存在直径为6或10 μ m的颗粒。我们的结果表明，在这些条件下，隔微血管的功能直径约为4 μ m，相邻角血管的直径略大，
To estimate the functional diameter of alveolar microvessels, we perfused isolated rat lungs with fluorescent latex particles (1 diameter/ lung) at inflation, pulmonary arterial, and left atrial pressures of 25, 30, and 0 cmH(2)O, respectively. We used confocal microscopy to count latex particles within septal microvessels and flow cytometry to count particle concentrations in venous outflow. We found 1-, 2-, and 4-mu m-diameter particles within septal vessels of 45 +/- 12, 31 +/- 12, and 25 +/- 9%, respectively, of examined alveoli. Particles of 5-mu m diameter were absent from septal vessels but were present within a small percentage of corner vessels. Particle concentrations in the venous outflow for 1-, 2-, 4-, and 5-mu m-diameter particles were 54 +/- 28, 67 +/- 32, 2.2 /- 0.3, and 0.4 +/- 0.3%, respectively, of the arterial inflow. Particles with diameters of 6 or 10 mu m were absent from venous outflow. Our results suggest that, under these conditions, the functional diameter of the septal microvessels is similar to 4 mu m and that the diameter of the adjacent corner vessels is slightly larger but