THE INVIVO REPLICATION ORIGIN OF THE YEAST 2-MU-M PLASMID
THE INVIVO REPLICATION ORIGIN OF THE YEAST 2-MU-M PLASMID
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DOI：
10.1016/0092-8674(87)90643-x
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发表时间：
1987-11-06
期刊：
影响因子：
64.5
通讯作者：
DAVIS, LR
中科院分区：
文献类型：
--
作者：
HUBERMAN, JA;SPOTILA, LD;DAVIS, LR
我们已经使用二维中性/碱性琼脂糖凝胶电泳根据复制程度将复制酵母2 μ m质粒DNA分子的新生链与非复制分子和亲本链分开。通过与短探针的顺序杂交分析新生链的长度表明，复制从图位3700 ± 1的单个起点双向进行。100，与基因定位的ARS元件一致。2 μ m质粒的两种重组异构体（A型和B型）以相同的效率复制。这些结果表明，ARS元件可能被证明是染色体DNA的复制起点。
We have used two-dimensional neutral/alkaline agarose gel electrophoresis to separate the nascent strands of replicating yeast 2.mu.m plasmid DNA molecules according to extent of replication, away from nonreplicating molecules and parental strands. Analysis of the lengths of nascent strands by sequential hybridization with short probes shows that replication proceeds bidirectionally from a single origin at map position 3700 .+-. 100, coincident with the genetically mapped ARS element. The two recombinational isomers of 2.mu.m plasmid (forms A and B) replicate with equal efficiency. These results suggest that ARS elements may prove to be replication origins for chromosomal DNA.