Phospholipase A activity in the hemolysis of sheep and human erythrocytes by Rickettsia prowazeki.
Phospholipase A activity in the hemolysis of sheep and human erythrocytes by Rickettsia prowazeki.
复制标题
普瓦泽基立克次体溶血羊和人红细胞中磷脂酶 A 的活性。
DOI：
10.1128/iai.29.2.316-321.1980
复制
发表时间：
1980
影响因子：
3.1
通讯作者：
Miller,ET
中科院分区：
文献类型：
--
作者：
Winkler,HH;Miller,ET
Incubation ofRickettsia prowazekiwith sheep or human erythrocytes resulted in lysis of the erythrocytes and formation of free fatty acids and lysophosphatides. Inhibitors of lysis were also invariably inhibitors of this phospholipase A activity. The target for this activity was the glycerophospholipids of the erythrocyte and not those of the rickettsia. Rickettsial phosphatidylethanolamine labeled with32PO4or [3H]acetate remained intact during lysis, and the composition of the free fatty acids released resembled that of the erythrocyte species used and not the rickettsiae. The products of hydrolysis remained associated with the sedimentable material in the reaction mixture under the usual conditions but partitioned into the supernatant fluid when bovine serum albumin was present. Initially, the time course of phospholipase A activity and lysis was identical, but the release of free fatty acids continued for a short time after the release of hemoglobin was complete. Both the inner and outer leaflets of the erythrocyte membrane were accessible to this rickettsial phospholipase A activity since both phosphatidylcholine and phosphatidylethanolamine were substrates in human erythrocytes. The questions of whether rickettsiae possess their own phospholipase A or activate a latent erythrocyte enzyme and what the role of the energy requirement is in these processes remain unanswered.
