Comparison of biotyping, ribotyping, and pulsed-field gel electrophoresis for investigation of a common-source outbreak of Burkholderia pickettii bacteremia
Comparison of biotyping, ribotyping, and pulsed-field gel electrophoresis for investigation of a common-source outbreak of Burkholderia pickettii bacteremia
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DOI：
10.1128/jcm.35.6.1398-1403.1997
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发表时间：
1997-06-01
影响因子：
9.4
通讯作者：
DeMol, P
中科院分区：
文献类型：
--
作者：
Chetoui, H;Melin, P;DeMol, P
在 3 个月的时间里，六名免疫功能低下的患者出现了一次或多次皮氏伯克霍尔德菌菌血症和/或导管感染，用于注射患者留置静脉装置的市售“无菌”注射盐水瓶被认为是微生物的共同来源，一旦停止使用这种盐水，就没有进一步诊断出病例。通过生物分型、EcoRI 和 HindIII 核糖分型以及 SpeI 脉冲场凝胶电泳 (PFGE) 对 26 株菌株进行了比较测试，其中包括来自病例患者和污染盐水的 9 株暴发相关菌株以及 17 株对照菌株。宏观限制分析显示了九个 PFGE 组，并且比核糖分型（七种核糖分型）和生物分型（两种生物变型）更具区分性。在与暴发相关的分离株中，通过三种分型方法发现了一种皮氏芽孢杆菌型。此外，PFGE 可用于细分核糖分型以及区分参与暴发的分离株与所有流行病学不相关的菌株。
Over a 3-month period, six immunocompromised patients developed one or more episodes of Burkholderia pickettii bacteremia and/or catheter infection, Vials of a commercially available, ''sterile'' saline for injection which had been used for Bushing the patients' indwelling intravenous devices were implicated as the common source of the organisms, No further cases were diagnosed once the use of this saline was discontinued. Twenty six isolates, including 9 outbreak-related strains from case patients and contaminated saline as well as 17 control strains, were tested comparatively by biotyping, ribotyping with EcoRI and HindIII, and pulsed-field gel electrophoresis (PFGE) with SpeI. Macrorestriction analysis revealed nine PFGE groups and was more discriminating than ribotyping (seven ribotypes) and biotyping (two biovars), Among the outbreak-related isolates, one B. pickettii type was found by the three typing methods, Furthermore, PFGE was useful for subdividing ribotypes and for distinguishing isolates involved in the outbreak from all epidemiologically unrelated strains.