Cyclic AMP in oocytes controls meiotic prophase I and primordial folliculogenesis in the perinatal mouse ovary
Cyclic AMP in oocytes controls meiotic prophase I and primordial folliculogenesis in the perinatal mouse ovary
复制标题
卵母细胞中的环磷酸腺苷控制围产期小鼠卵巢中的减数分裂前期 I 和原始卵泡发生。
DOI：
10.1242/dev.112755
复制
发表时间：
2015-01-15
期刊：
影响因子：
4.6
通讯作者：
Xia, Guoliang
中科院分区：
文献类型：
--
作者：
Wang, Yijing;Teng, Zhen;Xia, Guoliang
在哺乳动物的卵巢中，在围产期形成固定的原始卵泡群，并且包含在其中的卵母细胞在减数分裂前期I的网状体阶段被阻止。在目前的研究中，我们提供的证据表明，卵母细胞中的cAMP（cAMP）水平调节卵母细胞减数分裂前期I和原始卵泡发生在围产期小鼠卵巢。我们的研究结果表明，卵母细胞减数分裂早期的发展是密切相关的卵母细胞内cAMP水平的增加。抑制胎儿卵巢中cAMP的合成可延迟卵母细胞减数分裂进程，并抑制联会复合体蛋白1的分解和降解。此外，在体外培养的胎儿卵巢中抑制cAMP的合成阻止了原始卵泡的形成。最后，利用原位卵母细胞染色体分析的方法，我们发现，在生理条件下，卵母细胞的网状阻滞是原始卵泡形成所必需的。两者合计，这些结果表明，在小鼠卵巢中的早期减数分裂发育和原始卵泡形成的cAMP的作用。
In mammalian ovaries, a fixed population of primordial follicles forms during the perinatal stage and the oocytes contained within are arrested at the dictyate stage of meiotic prophase I. In the current study, we provide evidence that the level of cyclic AMP ( cAMP) in oocytes regulates oocyte meiotic prophase I and primordial folliculogenesis in the perinatal mouse ovary. Our results show that the early meiotic development of oocytes is closely correlated with increased levels of intra-oocyte cAMP. Inhibiting cAMP synthesis in fetal ovaries delayed oocyte meiotic progression and inhibited the disassembly and degradation of synaptonemal complex protein 1. In addition, inhibiting cAMP synthesis in in vitro cultured fetal ovaries prevented primordial follicle formation. Finally, using an in situ oocyte chromosome analysis approach, we found that the dictyate arrest of oocytes is essential for primordial follicle formation under physiological conditions. Taken together, these results suggest a role for cAMP in early meiotic development and primordial follicle formation in the mouse ovary.