INFLUENCE OF PH ON PORPHYRIN PRODUCTION IN PROPIONIBACTERIUM-ACNES
INFLUENCE OF PH ON PORPHYRIN PRODUCTION IN PROPIONIBACTERIUM-ACNES
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DOI：
10.1007/bf00413361
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发表时间：
1984-01-01
影响因子：
3
通讯作者：
SANDBERG, S
中科院分区：
文献类型：
--
作者：
KJELDSTAD, B;JOHNSSON, A;SANDBERG, S
痤疮丙酸杆菌在 Eagle's 培养基上、pH 5.3-7.2 下生长 4-15 天。通过直接荧光测定法和高压液相色谱法（HPLC）测量卟啉的产量。最大浓度的卟啉在 pH 6.1 时产生。原卟啉是所有样品中细菌中存在的主要卟啉种类。培养 4 天后，粪卟啉的相对量在 pH 6.7 时达到最大。人体皮肤的 pH 值存在局部差异；这些发现可能对于痤疮中卟啉的产生很重要。
P. acnes was grown on Eagle''s medium, for 4-15 days, at pH 5.3-7.2. The porphyrin production was measured both by direct fluorometry and by high pressure liquid chromatography (HPLC). The greatest concentration of porphyrins was produced at pH 6.1. Protoporphyrin was the dominant porphyrin species present in the bacteria in all samples. The relative amount of coproporphyrin was greatest at pH 6.7 after 4 days of incubation. In human skin, there are local variations in the pH; these findings may be of importance for porphyrin production in acne.