Biosynthesis of N-acetyl-L-aspartic acid in vivo and in brain homogenates.
Biosynthesis of N-acetyl-L-aspartic acid in vivo and in brain homogenates.
复制标题
N-乙酰基-L-天冬氨酸在体内和脑匀浆中的生物合成。
DOI：
10.1139/o70-034
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
M. Laliberté
中科院分区：
文献类型：
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作者：
L. Berlinguet;M. Laliberté
本文测定了胰腺切除犬和四氧嘧啶糖尿病大鼠尿中N-乙酰-L-天冬氨酸的含量。尿中N-乙酰-L-天冬氨酸经乙酸乙酯提取、水解后比色测定。结果表明，在四氧嘧啶处理的大鼠中，N-乙酰-L-天冬氨酸以相对高的浓度存在于尿液中，而在对照组中完全不存在。胰腺切除犬尿液中N-乙酰基-L-天冬氨酸的排泄量远高于正常犬。此外，当通过向脑匀浆中加入氟乙酸抑制柠檬酸循环时，培养基中存在放射性N-乙酰基-L-天冬氨酸的蓄积，而在对照中未检测到。结论：脑内N-乙酰-L-天冬氨酸的形成可以减轻过量乙酸的积累。这表明，N-乙酰基-L-天冬氨酸可以作为乙酰基的载体。
The content of N-acetyl-L-aspartic acid in urine from pancreatectomized dogs and alloxan-diabetic rats was measured. Urinary N-acetyl-L-aspartic acid was extracted by ethyl acetate and hydrolyzed, and the aspartic acid was determined by colorimetry. The results show that in alloxan-treated rats, N-acetyl-L-aspartic acid is present in relatively high concentration in urine, while it is completely absent in the controls. In pancreatectomized dogs, the excretion of N-acetyl-L-aspartic acid in urine is much higher than in normal dogs. Furthermore, when the citric acid cycle is inhibited by the addition of fluoroacetic acid to brain homogenates, there is accumulation of radioactive N-acetyl-L-aspartic acid in the medium whereas none could be detected in controls. It is concluded that the formation of N-acetyl-L-aspartic acid in the brain can alleviate the accumulation of an excess of acetate. It is suggested that N-acetyl-L-aspartic acid may serve as a carrier of the acetyl group.