Synthesis of highly porous crosslinked elastin hydrogels and their interaction with fibroblasts in vitro
Synthesis of highly porous crosslinked elastin hydrogels and their interaction with fibroblasts in vitro
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DOI：
10.1016/j.biomaterials.2009.05.014
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发表时间：
2009-09-01
期刊：
影响因子：
14
通讯作者：
Dehghani, Fariba
中科院分区：
文献类型：
--
作者：
Annabi, Nasim;Mithieux, Suzanne M.;Dehghani, Fariba
本研究探讨了利用高压CO2制备多孔弹性蛋白水凝胶的可行性。-弹性蛋白与六亚甲基二异氰酸酯化学交联，六亚甲基二异氰酸酯能与弹性蛋白中的各种官能团如赖氨酸、半胱氨酸和组氨酸发生反应。高压CO2实质上影响了制备的水凝胶的特性。当压力从1 bar增加到60 bar时，水凝胶的孔径增大了20倍。高压CO2法制备的样品溶胀率也高于常压法制备的凝胶。α -弹性蛋白水凝胶的压缩模量随着外加应变幅度从40%增加到80%而增加。高压CO2生成的水凝胶的压缩模量比常压条件下形成的凝胶低3倍，这是由于高压CO2生成的凝胶的孔隙度增加。在这些水凝胶的结构中制造了大孔隙，从而大大促进了细胞在整个基质中的渗透和生长。本研究制备的高孔弹性蛋白水凝胶结构在组织工程中具有潜在的应用前景。2009爱思唯尔有限公司版权所有。
In this study the feasibility of using high pressure CO2 to produce porous alpha-elastin hydrogels was investigated. alpha-Elastin was chemically crosslinked with hexamethylene diisocyanate that can react with various functional groups in elastin such as lysine, cysteine, and histidine. High pressure CO2 substantially affected the characteristics of the fabricated hydrogels. The pore size of the hydrogels was enhanced 20-fold when the pressure was increased from 1 bar to 60 bar. The swelling ratio of the samples fabricated by high pressure CO2 was also higher than the gels produced under atmospheric pressure. The compression modulus of alpha-elastin hydrogels was increased as the applied strain magnitude was modified from 40% to 80%. The compression modulus of hydrogels produced under high pressure CO2 was 3-fold lower than the gels formed at atmospheric conditions due to the increased porosity of the gels produced by high pressure CO2. The fabrication of large pores within the 31) structures of these hydrogels substantially promoted cellular penetration and growth throughout the matrices. The highly porous alpha-elastin hydrogel structures fabricated in this study have potential for applications in tissue engineering. (C) 2009 Elsevier Ltd. All rights reserved.