HLA expression and tumor-infiltrating immune cells in uveal melanoma
HLA expression and tumor-infiltrating immune cells in uveal melanoma
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DOI：
10.1007/bf00186516
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发表时间：
1996-01-01
影响因子：
2.7
通讯作者：
Jager, MJ
中科院分区：
文献类型：
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作者：
deWaardSiebinga, I;Hilders, CGJM;Jager, MJ
背景：在葡萄膜黑色素瘤中，肿瘤浸润细胞的数量和 HLA 抗原的表达水平变化很大。我们假设 HLA 表达水平低导致抗原呈递缺乏，这可能会妨碍肿瘤的正确免疫识别。这种识别的缺乏可能随后导致肿瘤浸润细胞水平较低。方法：为了检验这一假设，我们确定了 24 个葡萄膜黑色素瘤肿瘤切片中肿瘤浸润细胞的类型和数量。我们应用针对不同类型免疫细胞的单克隆抗体，并将结果与特纳细胞上 HLA I 类和 II 类抗原的表达进行比较。结果：在所有葡萄膜黑色素瘤中均观察到浸润性免疫细胞（尽管数量很少），其中以 T 淋巴细胞为主。 CD3+细胞（T淋巴细胞）的数量与单形HLA I类表达、等位基因特异性HLA-A2和Bw4表达以及HLA II类表达之间观察到显着正相关。此外，CD4+细胞（T辅助细胞、单核细胞/巨噬细胞）和CD11b+细胞（单核细胞/巨噬细胞）的数量与单形HLA I类表达水平显着相关。结论：这些数据支持我们的假设，即低水平的 HLA 表达（因此缺乏肿瘤特异性抗原的呈递）可能导致低水平的肿瘤浸润。
Background: In uveal melano ma, both the amount of tumor-infiltrating cells and the level of expression of HLA antigens are quite variable. We hypothesized that low levels of HLA expression lead to a lack of antigen presentation, which might prevent proper immunologic recognition of the tumor. This lack of recognition might subsequently lead to low levels of tumor-infiltrating cells. Methods: To test this hypothesis, we determined the type and number of tumor-infiltrating cells in tumor sections from 24 uveal melanomas. We applied monoclonal antibodies directed against different types of immune cells and compared the results with the expression of HLA class I and class II antigens on the turner cells. Results: Infiltrating immune cells were observed in all uveal melanomas (although in small amounts), with a predominance of T lymphocytes. Significant positive correlation:, were observed between the number of CD3+ cells (T lymphocytes) and monomorphic HLA class I expression, allele-specific HLA-A2 and Bw4 expression, and HLA class II expression. Furthermore, the number of CD4+ cells (T helper cells, monocytes/macrophages) and of CD11b+ cells (monocytes/macrophages) was significantly correlated with the level of monomorphic HLA class I expression. Conclusion: These data support our hypothesis that low levels of HLA expression (and therefore a lack of presentation of tumor-specific antigens) may lead to a low level of tumor infiltrate.