Alteration of carotid body chemoreflexes after neonatal intermittent hypoxia and caffeine treatment in rat pups
Alteration of carotid body chemoreflexes after neonatal intermittent hypoxia and caffeine treatment in rat pups
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DOI：
10.1016/j.resp.2011.05.006
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发表时间：
2011-08-15
影响因子：
2.3
通讯作者：
Bairam, Aida
中科院分区：
文献类型：
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作者：
Julien, Cecile A.;Joseph, Vincent;Bairam, Aida
在人类新生儿中，咖啡因治疗早产儿呼吸暂停，特别是与低氧血症相关时，在出生后维持数周。在本研究中，我们使用新生大鼠和全身体积描记术来测试单独或联合新生儿间歇性缺氧（n-IH）慢性暴露于新生儿咖啡因治疗（Na）是否改变：（1）基线通气和对缺氧的反应（2）对急性腹膜内注射柠檬酸咖啡因（20 mg/kg）或多潘立酮（外周多巴胺D2受体拮抗剂）（1 mg/kg）的反应。对四组大鼠进行了如下研究：在正常条件下饲养，每天灌胃水（NWT）或Na，或从出生后第3天至第12天暴露于nIH（n-IH + NWT和n-IH + NCT）。在n-IH + NCT大鼠中，基线通气量高于其他组。咖啡因或多潘立酮仅在NWT和n-IH + NWT大鼠中增强基线通气，但咖啡因或多潘立酮均不影响这些组中的低氧通气反应。在n-IH + NWT大鼠中，缺氧早期的反应（
In human neonates, caffeine therapy for apnoea of prematurity, especially when associated with hypoxemia, is maintained for several weeks after birth. In the present study, we used newborn rats and whole-body plethysmography to test whether chronic exposure to neonatal caffeine treatment (Na), alone or combined with neonatal intermittent hypoxia (n-IH) alters: (1) baseline ventilation and response to hypoxia (12% 02,20 min); and (2) response to acute i.p. injection of caffeine citrate (20 mg/kg) or domperidone, a peripheral dopamine D2 receptor antagonist (1 mg/kg). Four groups of rats were studied as follows: raised under normal conditions with daily gavage with water (NWT) or Na, or exposed to nIH (n-IH + NWT and n-IH + NCT) from postnatal days 3 to 12. In n-IH + NCT rats, baseline ventilation was higher than in the other groups. Caffeine or domperidone enhanced baseline ventilation only in NWT and n-IH + NWT rats, but neither caffeine nor domperidone affected the hypoxic ventilatory response in these groups. In n-IH + NWT rats, the response during the early phase of hypoxia (