CD133 expression in osteosarcoma and derivation of CD133+ cells
CD133 expression in osteosarcoma and derivation of CD133+ cells
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骨肉瘤中 CD133 的表达及 CD133 细胞的来源
DOI：
10.3892/mmr.2012.1231
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发表时间：
2013-02-01
影响因子：
3.4
通讯作者：
Liu, Wei
中科院分区：
文献类型：
--
作者：
Li, Ji;Zhong, Xiao-Yan;Liu, Wei
分化簇133（CD 133）被认为是正常组织和癌组织的干细胞标志物。采用细胞培养和实时荧光聚合酶链反应，分析CD 133在骨肉瘤组织和Saos-2细胞系中的表达。此外，我们还检测了Saos-2细胞系中肿瘤干细胞相关基因的表达，以探讨骨肉瘤发生和高耐药的机制。CD 133（+）细胞在骨肉瘤组织中广泛分布。细胞培养后从G（0）/G（1）进入G（2）/M和S细胞周期阶段。在细胞培养过程中，CD 133（+）细胞水平迅速下降至正常水平。Saos-2细胞的CD 133（+）亚群的集落形成效率高于CD 133（-）亚群。干细胞相关基因的表达水平，包括多药耐药蛋白1（MDR 1）和性别决定区Y盒2（Sox 2）在CD 133（+）细胞亚群中被发现显着高于CD 133（-）亚群。这些观察结果表明，CD 133（+）Saos-2细胞表现出干细胞特征，包括低丰度、静止和高分化潜力，以及关键干细胞调控和耐药基因的表达，这可能导致骨肉瘤和高耐药性。
Cluster of differentiation 133 (CD133) is recognized as a stem cell marker for normal and cancerous tissues. Using cell culture and real-time fluorescent polymerase chain reaction, CD133 expression was analyzed in osteosarcoma tissue and Saos-2 cell lines. In addition, cancer stem cell-related gene expression in the Saos-2 cell line was determined to explore the mechanisms underlying tumorigenesis and high drug resistance in osteosarcoma. CD133(+) cells were found to be widely distributed in various types of osteosarcoma tissue. Following cell culture, cells entered the G(2)/M and S cell cycle stages from G(0)/G(1). Levels of CD133(+) cells decreased to normal levels rapidly over the course of cell culture. Colony forming efficiency was higher in the CD133(+) compared with the CD133(-) subpopulation of Saos-2 cells. Expression levels of stem cell-related genes, including multidrug resistance protein 1 (MDR1) and sex determining region Y-box 2 (Sox2) in the CD133(+) subpopulation of cells were found to be significantly higher compared with the CD133(-) subpopulation. These observations indicate that CD133(+) Saos-2 cells exhibit stem cell characteristics, including low abundance, quiescence and a high potential to undergo differentiation, as well as expression of key stem cell regulatory and drug resistance genes, which may cause osteosarcoma and high drug resistance.