The cognitive impairment induced by zinc deficiency in rats aged 0≈2 months related to BDNF DNA methylation changes in the hippocampus
The cognitive impairment induced by zinc deficiency in rats aged 0≈2 months related to BDNF DNA methylation changes in the hippocampus
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DOI：
10.1080/1028415x.2016.1194554
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发表时间：
2017-01-01
影响因子：
3.6
通讯作者：
Jiang, Yu-Gang
中科院分区：
文献类型：
--
作者：
Hu, Yan-Dan;Pang, Wei;Jiang, Yu-Gang
目的：本研究旨在探讨缺锌对0 ~ 2月龄大鼠学习记忆能力的影响及对海马脑源性神经营养因子（BDNF）基因甲基化状态的影响（ZA：锌30 mg/kg饲料）、缺锌组（ZD：锌1 mg/kg饲料）和配对喂养组（PF：锌30 mg/kg饲料）。断奶后（第23天），后代喂食与其母亲相同的饮食。37 d后测定仔鼠血浆和海马锌浓度，采用被动回避行为测试法测定仔鼠行为功能。采用甲基化特异性实时定量PCR检测BDNF基因外显子IX的甲基化模式，采用RT-PCR检测海马BDNF mRNA的表达。结果：与ZA组和PF组相比，ZD组大鼠的潜伏期缩短，血浆和海马锌含量降低（P
Objective: This study was carried out to understand the effects of zinc deficiency in rats aged 0 approximate to 2 months on learning and memory, and the brain-derived neurotrophic factor (BDNF) gene methylation status in the hippocampus.Methods: The lactating mother rats were randomly divided into three groups (n=12): zinc-adequate group (ZA: zinc 30mg/kg diet), zinc-deprived group (ZD: zinc 1mg/kg diet), and a pair-fed group (PF: zinc 30mg/kg diet), in which the rats were pair-fed to those in the ZD group. After weaning (on day 23), offspring were fed the same diets as their mothers. After 37 days, the zinc concentrations in the plasma and hippocampus were measured, and the behavioral function of the offspring rats was measured using the passive avoidance performance test. We then assessed the DNA methylation patterns of the exon IX of BDNF by methylation-specific quantitative real-time PCR and the mRNA expression of BDNF in the hippocampus by RT-PCR.Results: Compared with the ZA and PF groups, rats in the ZD group had shorter latency period, lower zinc concentrations in the plasma and hippocampus (P