kasT gene of Streptomyces kasugaensis M338-M1 encodes a DNA-binding protein which binds to intergenic region of kasU-kasJ in the kasugamycin biosynthesis gene cluster.
kasT gene of Streptomyces kasugaensis M338-M1 encodes a DNA-binding protein which binds to intergenic region of kasU-kasJ in the kasugamycin biosynthesis gene cluster.
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DOI：
10.7164/antibiotics.55.1053
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发表时间：
2002-12
期刊：
影响因子：
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通讯作者：
S. Ikeno;D. Aoki;Koji Sato;M. Hamada;M. Hori;K. Tsuchiya
中科院分区：
文献类型：
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作者：
S. Ikeno;D. Aoki;Koji Sato;M. Hamada;M. Hori;K. Tsuchiya
我们曾报道过春雷霉素（KSM）产生菌春日霉素链霉菌M338-M1的4.2kb SacI-EcoRI DNA区域中含有KSM转运蛋白基因（kasKLM）。作为该研究的延伸，克隆并测序了位于kasK上游1.5约5.2 kb处的3.7 kb Psti-SacI DNA区域，揭示了三个完整的开放阅读框架，命名为kasT、kasU和kasJ。kasJ基因编码一个具有保守的二核苷酸（FAD）结合基序的蛋白（KasJ）。灰色的kasT基因编码一种DNA结合蛋白（KasT），在序列中心附近包含一个螺旋-转角-螺旋基序。该蛋白质在结构上类似于在调节S.灰色的融合蛋白（Trx-KasT）清楚地显示出与kasU-kasJ基因间区域的DNA结合活性，表明KasT是KSM生物合成基因簇的途径特异性调节剂。
We previously reported that a 4.2 kb SacI-EcoRI DNA region from Streptomyces kasugaensis M338-M1, a kasugamycin (KSM) producer, included KSM transporter genes (kasKLM). As an extension of that study, a 3.7 kb Psti-SacI DNA region, located at 1.5 approximately 5.2 kb upstream of kasK, was cloned and sequenced, revealing three complete open reading frames, designated kasT, kasU and kasJ. The kasJ gene encodes a protein (KasJ) with a conserved dinucleotide (FAD)-binding motif Homology search for KasJ showed its similarity to NADH: N-amidino-scyllo-inosamine oxidoreductase (StsB) which is involved in biosynthesis of the streptidine moiety of streptomycin (SM) in S. griseus. The kasT gene encodes a DNA-binding protein (KasT), including a helix-turn-helix motif near the center of the sequence. This protein is similar in structure to a pathway-specific activator protein (StrR) that plays a role in regulating the SM biosynthesis gene cluster of S. griseus. A fusion protein (Trx-KasT) clearly showed DNA binding activity with the intergenic region of kasU-kasJ, suggesting that KasT is a pathway-specific regulator of the KSM biosynthesis gene cluster.