A colorimetric method for the measurement of ppb-level NO in exhaled air using porous glass analytical chips
A colorimetric method for the measurement of ppb-level NO in exhaled air using porous glass analytical chips
复制标题
使用多孔玻璃分析芯片测量呼出气体中 ppb 级 NO 的比色法
DOI：
10.1016/j.snr.2020.100019
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发表时间：
2020
影响因子：
5.9
通讯作者：
Maruo Yasuko Y.
中科院分区：
文献类型：
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作者：
Asanuma Kohgo;Numata Keita;Maruo Yasuko Y.
哮喘患者呼出高浓度的一氧化氮(NO)，因此NO的检测具有重要的临床意义。尽管我们之前开发了一种2-phenyl-4，4，5，5-tetramethylimidazoline-3-oxide-1-oxyl芯片，它依赖于芯片中NO气体和PTIO之间的反应，但对于哮喘患者呼出的空气中NO的测量，灵敏度和准确度都不够高。在这项研究中，我们重点研究了在PTIO反应中NO转化为NO2的反应，并设计了一种新的方法来测量转化后的NO2，使用我们之前开发的NO2分析芯片。在PTIO芯片中，NO被转化为NO2，并在暴露在大气中时立即释放。这释放了捕获在相邻的NO2分析芯片上的NO2 IS，并与重氮偶联剂反应生成偶氮染料；NO浓度与由于偶氮染料的产生而在525 nm处的吸光度变化呈线性关系。用设计的方法可以在0−142ppb范围内测量NO浓度，在142 ppb时最大误差为±10ppb，表明该方法具有足够的灵敏度和精确度来测量呼出空气中的NO。此外，我们成功地检测了7名健康成人呼出空气中的NO，检测到~19 ppb的NO，从而证明了其临床应用的可行性。
Patients with asthma exhale high concentrations of nitric oxide (NO), making the measurement of NO clinically important. Although we previously developed a 2-phenyl-4,4,5,5-tetramethylimidazoline-3-oxide-1-oxyl (PTIO) chip that relied on the reaction between NO gas and PTIO in the chip, the sensitivity and accuracy were insufficient for measuring NO in air exhaled by asthma patients. In this study, we focus on the conversion of NO to NO2in the reaction with PTIO, and devise a new method to measure the converted NO2using our previously developed NO2analytical chip. NO is converted to NO2in the PTIO chip and immediately released upon exposure to the atmosphere. This released NO2is trapped on the adjacent NO2analytical chip and reacts with diazo-coupling reagents to form an azo dye; a linear relationship was identified between the NO concentration and the change in absorbance at 525 nm due to production of the azo dye. The NO concentration could be measured in the 0−142 ppb range with a maximum error of ± 10 ppb at 142 ppb using the devised method, demonstrating sufficient sensitivity and accuracy for measuring NO in exhaled air. Furthermore, we successfully measured NO in the exhaled air of seven healthy adults, and detected ~19 ppb of NO, thus illustrating its feasibility for clinical use.