Enantioselective analysis of D- and L-amino acids from mouse macrophages using high performance liquid chromatography
Enantioselective analysis of D- and L-amino acids from mouse macrophages using high performance liquid chromatography
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DOI：
10.1016/j.jpba.2015.04.028
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发表时间：
2015-12-10
影响因子：
3.4
通讯作者：
Oikawa, Tadao
中科院分区：
文献类型：
--
作者：
Kato, Shiro;Masuda, Yuki;Oikawa, Tadao
采用高效液相色谱法分析了从腹腔提取的小鼠巨噬细胞的固有D-氨基酸谱。在小鼠巨噬细胞裂解液中检测到6种D-氨基酸（D-Asp、D-Ser、D-Ala、D-Leu、D-Gln和D-Lys）。含量和D/D + L比根据D-氨基酸的类型而不同，分别为约3.5-22 nmol/g细胞和约1- 20%。模型巨噬细胞系RAW 264.7细胞的D-氨基酸组成与小鼠巨噬细胞的D-氨基酸组成相似。这些结果表明，巨噬细胞和具有巨噬细胞样功能的RAW 264.7细胞具有相似的D-氨基酸谱。(C)2015 Elsevier B. V.版权所有。
The intrinsic D-amino acid profile of mouse macrophages extracted from the peritoneal cavity was analyzed using high performance liquid chromatography. Six D-amino acids (D-Asp, D-Ser, D-Ala, D-Leu, D-Gln and D-Lys) were detected in cell lysates of mouse macrophages. The content and the D/D + L ratio differed depending on the type of D-amino acid and were approximately 3.5-22 nmol/g cells, and approximately 1-20%, respectively. The D-amino acid composition of RAW 264.7 cells, which is a model macrophage cell line, was similar to that of the mouse macrophage. These results suggest that macrophages and RAW 264.7 cells with macrophage-like functions have a similar D-amino acid profile. (C) 2015 Elsevier B.V. All rights reserved.