Binding to the yeast Swi4,6-dependent cell cycle box, CACGAAA, is cell cycle regulated in vivo
Binding to the yeast Swi4,6-dependent cell cycle box, CACGAAA, is cell cycle regulated in vivo
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DOI：
10.1093/nar/24.4.558
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发表时间：
1996-02-15
影响因子：
14.9
通讯作者：
Andrews, BJ
中科院分区：
文献类型：
--
作者：
Harrington, LA;Andrews, BJ
承诺在酿酒酵母细胞分裂发生在细胞周期G1期的点称为启动和需要的活动Cdc28蛋白激酶及其相关的细胞周期蛋白,G1 Swi4, 6-dependent细胞周期因素框绑定,SBF,是重要的最大G1细胞周期蛋白和HO核酸内切酶基因的表达开始,这些基因的细胞周期调控是通过一个上游监管元素称为调制渣打银行(Swi4, 6-dependent细胞周期盒子,CACGAAA)依赖SWI4和SWI6虽然绑定SWI4和SWI6渣打银行序列已经研究的很透彻了体外,绑定的SBF体内没有检查,我们使用体内硫酸二甲酯碳足迹研究渣打银行序列的入住率在整个细胞周期,我们发现绑定到渣打银行序列发生在细胞周期G1期,在G2大大减少,在缺乏SWI4或者SWI6,SCB序列在细胞周期的任何阶段都没有被占用，这些结果表明，体内SCB依赖基因的g1特异性表达在DNA结合水平上受到调节。
In Saccharomyces cerevisiae commitment to cell division occurs late in the G1 phase of the cell cycle at a point called Start and requires the activity of the Cdc28 protein kinase and its associated G1 cyclins, The Swi4,6-dependent cell cycle box binding factor, SBF, is important for maximal expression of the G1 cyclin and HO endonuclease genes at Start, The cell cycle regulation of these genes is modulated through an upstream regulatory element termed the SCB (Swi4,6-dependent cell cycle box, CACGAAA), which is dependent on both SWI4 and SWI6, Although binding of Swi4 and Swi6 to SCB sequences has been well characterized in vitro, the binding of SBF in vivo has not been examined, We used in vivo dimethyl sulfate footprinting to examine the occupancy of SCB sequences throughout the cell cycle, We found that binding to SCB sequences occurred in the G1 phase of the cell cycle and was greatly reduced in G2, In the absence of either Swi4 or Swi6, SCB sequences were not occupied at any cell cycle stage, These results suggest that the G1-specific expression of SCB-dependent genes is regulated at the level of DNA binding in vivo.