Cold-spray coating of hydroxyapatite on a three-dimensional polyetheretherketone implant and its biocompatibility evaluated by in vitro and in vivo minipig model
Cold-spray coating of hydroxyapatite on a three-dimensional polyetheretherketone implant and its biocompatibility evaluated by in vitro and in vivo minipig model
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DOI：
10.1002/jbm.b.33589
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发表时间：
2017-04-01
影响因子：
3.4
通讯作者：
Noh, Jun Hong
中科院分区：
文献类型：
--
作者：
Lee, Jae Hyup;Jang, Hae Lin;Noh, Jun Hong
PEEK是一种生物惰性材料，不会与天然骨组织发生化学结合，因此在PEEK上形成天然骨样羟基磷灰石（HA）涂层是改善PEEK生物相容性和保持其机械性能的重要挑战。在各种涂层技术中，冷喷涂法适合在PEEK上形成稳定的HA涂层，同时保持其化学性能，因为它可以在相对较低的温度范围内进行。因此，在本研究中，我们使用冷喷涂方法在表面具有周期性脊的地形复杂的PEEK基底上形成一层厚的HA，并植入与人体系统相似的小型猪髂骨缺损中。此外，将临床使用的PEEK融合器涂有HA并插入小型猪的腰椎间盘间隙中。我们在体外试验中观察到HA涂层PEEK植入物上的人骨髓间充质干细胞的ALP活性、钙生成和BSP生成高于裸PEEK组。此外，二维组织学分析和三维微型CT分析表明，将复杂形状的HA-PEEK混合植入物植入体内小型猪模型中，具有足够的生物相容性和骨整合性，可用于进一步的临床应用。值得注意的是，由于HA涂层增强了PEEK椎间融合器的稳定性，PEEK椎间融合器内装载的小HA块的骨整合率也显著提高，这表明HA涂层PEEK椎间融合器的融合率和粘附性总体增加。(c)2015 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，105 B：647-657，2017。
PEEK is a bioinert material that does not chemically bind to native bone tissue and thus formation of natural bone-like hydroxyapatite (HA) coating layer on PEEK has been an important challenge to improve biocompatibility and to preserve mechanical property of PEEK. Among various coating techniques, cold-spray coating method is suitable to form stable HA coating layer on PEEK while maintaining their chemical properties, because it can be conducted in relatively low-temperature range. Therefore, in this research, we used cold-spray coating method to form a thick layer of HA on the topographically complex PEEK substrates with periodic ridges on the surface and implanted in iliac bone defects of minipigs which is known to be similar with human body system. In addition, PEEK cage for clinical usage was coated with HA and inserted in the lumbar intervertebral disc space of minipig. We observed higher ALP activity, calcium production, and BSP production of human bone marrow mesenchymal stem cells on the HA-coated PEEK implants than the bare PEEK group in in vitro test. In addition, two-dimensional histological analysis and three-dimensional micro CT analysis demonstrated that implantation of complex shape of HA-PEEK hybrid implant in in vivo minipig model resulted sufficient biocompatibility and osseointegration for further clinical applications. Notably, due to the enhanced stability of PEEK cage induced from HA coating layer, osseointegration rate of the small HA blocks loaded inside the PEEK cage was also significantly improved which indicates overall increased fusion rate and adherence of the HA-coated PEEK cage. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 647-657, 2017.