Isolation of Chlamydia psittaci from naturally infected African clawed frogs (Xenopus laevis).
Isolation of Chlamydia psittaci from naturally infected African clawed frogs (Xenopus laevis).
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从自然感染的非洲爪蛙（Xenopus laevis）中分离出鹦鹉热衣原体。
DOI：
10.1128/iai.41.2.789-794.1983
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发表时间：
1983
影响因子：
3.1
通讯作者：
Nace,GW
中科院分区：
文献类型：
--
作者：
WilckeJr,BW;Newcomer,CE;Anver,MR;Simmons,JL;Nace,GW
从商业饲养的非洲爪蛙（Xenopus laevis）的肝脏中分离出一种包涵体形成剂，该蛙涉及一种高发病率和死亡率的流行病。最初的分离是在 McCoy 细胞中进行的。根据典型胞质内包涵体的形成，该病原体被鉴定为鹦鹉热衣原体，该包涵体在 48 小时内发育，不被碘染色，并且对磺胺嘧啶具有抗性。进一步研究了从一种特定青蛙（指定为菌株 178）中分离出来的菌株，发现其对于卵黄囊内接种后 7 天大的含胚鸡蛋具有致死性。该菌株被证明对小鼠腹膜内接种时不具有致病性。鹦鹉热衣原体的细胞培养分离物被传播给未感染的非洲角杆菌，导致疾病和死亡。
An inclusion-forming agent was isolated from the livers of commercially raised African clawed frogs (Xenopus laevis) involved in an epizootic of high morbidity and mortality. Original isolation was made in McCoy cells. This agent was identified as Chlamydia psittaci based on the formation of typical intracytoplasmic inclusions which developed within 48 h, were not stained by iodine, and were resistant to sulfadiazine. The isolate from one particular frog (designated as strain 178) was further studied and found to be lethal for 7-day-old embryonated chicken eggs after intra-yolk sac inoculation. This strain was demonstrated not to be pathogenic for mice when inoculated intraperitoneally. The cell culture isolate of C. psittaci was transmitted to uninfected X. laevis, causing disease and death.