Methoxyquinoline-diethylenetriamine conjugate as a fluorescent zinc sensor
Methoxyquinoline-diethylenetriamine conjugate as a fluorescent zinc sensor
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DOI：
10.1039/c0dt00791a
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发表时间：
2011-01-01
影响因子：
4
通讯作者：
Tamotsu, Satoshi
中科院分区：
文献类型：
--
作者：
Mikata, Yuji;Yamashita, Azusa;Tamotsu, Satoshi
合成了一种6-甲氧基喹啉共轭二乙烯三胺衍生物N，N“-双（6-甲氧基-2-喹啉甲基）二乙烯三胺（6-MeOBQDIEN），并研究了其对锌离子的荧光响应。在锌离子存在下，6-MeOBQDIEN显示荧光（λ（ex）= 329 nm，λ（em）= 418 nm，phi = 0.039）。在相同条件下，该化合物的锌配合物的荧光强度比母体BQDIEN（phi = 0.021）高出两倍。6-MeOBQDIEN-Zn配合物的晶体结构表明，所有五个氮原子都以扭曲的四方锥几何构型（τ = 0.145）参与金属配位，其中脂肪族氮位于顶端位置。使用6-MeOBQDIEN的荧光显微镜分析揭示了活细胞中锌离子浓度的变化。
A 6-methoxyquinoline conjugated diethylenetriamine derivative, N,N ''-bis(6-methoxy-2-quinolylmethyl) diethylenetriamine (6-MeOBQDIEN) has been synthesized and its fluorescent response toward zinc ion was investigated. In the presence of zinc ion, 6-MeOBQDIEN exhibits fluorescence (lambda(ex) = 329 nm, lambda(em) = 418 nm, phi = 0.039). The fluorescent intensity of the zinc complex of the compound is two times higher than the parent BQDIEN (phi = 0.021) under the same conditions. The crystal structure of 6-MeOBQDIEN-Zn complex shows that all five nitrogen atoms participate to the metal coordination in a distorted square-pyramidal geometry (tau = 0.145) with the aliphatic nitrogen in an apical position. Fluorescent microscopic analysis using 6-MeOBQDIEN reveals the zinc ion concentration change in living cells.