Cell-Penetrating Peptide as a Means of Directing the Differentiation of Induced-Pluripotent Stem Cells.
Cell-Penetrating Peptide as a Means of Directing the Differentiation of Induced-Pluripotent Stem Cells.
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DOI：
10.3390/ijms161125986
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发表时间：
2015-11-06
影响因子：
5.6
通讯作者：
Tomizawa K
中科院分区：
文献类型：
--
作者：
Kaitsuka T;Tomizawa K
使用细胞穿透肽（CPP）的蛋白质转导可用于递送大蛋白质分子，包括一些转录因子。该方法比用病毒载体的基因转染方法更安全，因为不存在外源DNA的基因组整合的风险。最近，这种方法被报道为诱导多能干细胞（iPS）的一种手段，指导分化为特定的细胞类型，并支持基因编辑/校正。此外，我们开发了一种直接分化方法，通过三种转录因子Pdx 1，NeuroD和MafA的蛋白转导从小鼠和人多能干细胞获得胰腺谱系。在这里，我们讨论了使用CPP作为指导iPS细胞和其他干细胞技术分化的手段的可能性。
Protein transduction using cell-penetrating peptides (CPPs) is useful for the delivery of large protein molecules, including some transcription factors. This method is safer than gene transfection methods with a viral vector because there is no risk of genomic integration of the exogenous DNA. Recently, this method was reported as a means for the induction of induced pluripotent stem (iPS) cells, directing the differentiation into specific cell types and supporting gene editing/correction. Furthermore, we developed a direct differentiation method to obtain a pancreatic lineage from mouse and human pluripotent stem cells via the protein transduction of three transcription factors, Pdx1, NeuroD, and MafA. Here, we discuss the possibility of using CPPs as a means of directing the differentiation of iPS cells and other stem cell technologies.