16 Y chromosomal STR haplotypes in Japanese
16 Y chromosomal STR haplotypes in Japanese
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DOI：
10.1016/j.forsciint.2007.01.032
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发表时间：
2008-01-15
影响因子：
2.2
通讯作者：
Kasai, Kentaro
中科院分区：
文献类型：
--
作者：
Mizuno, Natsuko;Nakahara, Hiroaki;Kasai, Kentaro
共对1079名日本男性进行了以下16种Y染色体短串联重复序列（Y-STR）标记的分型：DYS 456、DYS 389 I、DYS 390、DYS 389 II、DYS 458、DYS 19、DYS 385、DYS 393、DYS 391、DYS 439、DYS 635、DYS 392、Y加塔H4、DYS 437、DYS 438和DYS 448，使用AmpFISTR（R）YfilerTM PCR扩增试剂盒（Applied Biosystems）。共检测到16个Y-STR标记的950个单倍型，其中886个单倍型是独特的。在22名日本男性中发现了最常见的单倍型。单倍型多样性为0.9992，表明区分男性个体的潜力很大。在DYS 448标记处有10种单倍型未检测到等位基因。因此，当对来自一个以上男性个体的降解DNA样品和/或DNA混合物样品获得的DNA分型结果进行解释时，应注意此类非典型单倍型的存在。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
A total of 1079 Japanese males were typed for the following 16 Y chromosomal short tandem repeat (Y-STR) markers: DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385, DYS393, DYS391, DYS439, DYS635, DYS392, Y GATA H4, DYS437, DYS438 and DYS448 using an AmpFISTR (R) Yfiler (TM) PCR Amplification kit (Applied Biosystems). A total of 950 haplotypes for the 16 Y-STR markers were detected and, of these, 886 haplotypes were unique. The most frequent haplotype was found in 22 Japanese males. The haplotype diversity was 0.9992, indicating a high potential for differentiating between male individuals. There were 10 haplotypes with no allele detected at the DYS448 marker. Thus, the presence of such atypical haplotypes should be noted, when DNA typing results obtained from degraded DNA samples and/or DNA mixture samples from more than one male individual are being interpreted. (c) 2007 Elsevier Ireland Ltd. All rights reserved.