Culturable heterotrophic bacteria in seawater and Mytilus galloprovincialis from a Mediterranean area (Northern Ionian Sea-Italy)
Culturable heterotrophic bacteria in seawater and Mytilus galloprovincialis from a Mediterranean area (Northern Ionian Sea-Italy)
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DOI：
10.1007/s10661-008-0223-8
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发表时间：
2009-02-01
影响因子：
3
通讯作者：
Stabili, L.
中科院分区：
文献类型：
--
作者：
Cavallo, R. A.;Acquaviva, M. I.;Stabili, L.
虽然意大利爱奥尼亚海塔兰托的Mar Piccolo是最重要的贻贝养殖区之一，但关于这些贻贝及其周围环境的自然细菌微生物区系的数据仍然很少。这项研究是季节性地进行的，历时一年，目的是确定在北爱奥尼亚海的三个采样点(S.Vito、Lido Gandoli和Lido Silvana)采集的水和贻贝样品中的可培养异养菌。用海洋琼脂平板涂布法测定可培养异养菌丰度。通过形态、培养和生化方法对异养菌进行了鉴定。与全年相应的海水相比，贻贝样本中的细菌浓度更高。在革兰氏阴性异养菌中，气单胞菌在水体(18%)和贻贝(40%)样品中均占优势。其他属如莫拉氏菌属、假单胞菌属、产碱杆菌属、不动杆菌属、黄杆菌属、变色杆菌属、发光菌属和柔性杆菌属的分离率不同。革兰氏阳性菌以杆菌为主。一些属(卢杆菌和弧菌)只存在于贻贝样品中。这些结果有助于提高人们对北爱奥尼亚海贻贝及其周围海水中细菌丰度和多样性的认识。
Although Mar Piccolo of Taranto (Ionian Sea, Italy) is one of the most important Mytilus galloprovincialis farming areas, data concerning the natural bacterial microbiota of these mussels and their surrounding environment are still scant. This study was carried out seasonally, throughout a year, to determine culturable heterotrophic bacteria both in the water and mussels samples collected at three sampling sites in the Northern Ionian Sea: S. Vito, Lido Gandoli and Lido Silvana. Culturable heterotrophic bacteria abundance was determined by spread plate on Marine Agar. Heterotrophic bacteria were identified by several morphological, culture and biochemical methods. Bacterial concentrations were higher in the mussel samples compared to the corresponding seawater throughout the year. Among Gram negative heterotrophic bacteria, Aeromonas prevailed both in the water (18%) and mussel samples (40%). Other genera such as Moraxella, Pseudomonas, Alcaligenes, Acinetobacter, Flavobacterium, Chromobacterium, Photobacterium and Flexibacter were present with different percentages of isolation. Bacilli were predominant among Gram positive bacteria. Some genera (Lucibacterium and Vibrio) were present only in mussel samples. The results obtained contribute to improve the knowledge on both the bacterial abundance and diversity in mussels and the surrounding seawater in the Northern Ionian Sea.