Simple histidine-rich protein 2 double-site sandwich enzyme-linked immunosorbent assay for use in malaria drug sensitivity testing
Simple histidine-rich protein 2 double-site sandwich enzyme-linked immunosorbent assay for use in malaria drug sensitivity testing
复制标题
DOI：
10.1128/aac.49.8.3575-3577.2005
复制
发表时间：
2005-08-01
影响因子：
4.9
通讯作者：
Fukuda, M
中科院分区：
文献类型：
--
作者：
Noedl, H;Bronnert, J;Fukuda, M
建立了一种基于富组氨酸蛋白2（HRP 2）检测的双位点夹心酶联免疫吸附试验（ELISA）。ELISA使用两种商业单克隆抗体，并提供了一个大大便宜的替代试剂盒，以前使用的HRP 2药物敏感性测试。该测定法易于建立和执行。灵敏度相当，药物敏感性结果与商业ELISA试剂盒获得的结果非常接近（R-2 = 0.979; P < 0.001; 50%抑制浓度下的平均对数差= 0.07）。
A simple double-site sandwich enzyme-linked immunosorbent assay (ELISA) for Plasmodium falciparum in vitro drug sensitivity tests based on measuring histidine-rich protein 2 (HRP2) is presented. The ELISA uses two commercial monoclonal antibodies and provides a drastically cheaper alternative to the test kits previously used in the HRP2 drug sensitivity test. The assay is simple to establish and perform. The sensitivity is comparable and the drug sensitivity results very closely match those obtained with the commercial ELISA kits (R-2 = 0.979; P < 0.001; mean log difference at the 50% inhibitory concentration = 0.07).