Heat shock factor gene family in rice: Genomic organization and transcript expression profiling in response to high temperature, low temperature and oxidative stresses
Heat shock factor gene family in rice: Genomic organization and transcript expression profiling in response to high temperature, low temperature and oxidative stresses
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DOI：
10.1016/j.plaphy.2009.05.003
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发表时间：
2009-09-01
影响因子：
6.5
通讯作者：
Grover, Anil
中科院分区：
文献类型：
--
作者：
Mittal, Dheeraj;Chakrabarti, Sveta;Grover, Anil
热休克因子(HSFs)与热休克元件序列的结合是热休克基因转录诱导的关键。水稻基因组序列显示26个OsHsf基因，其中25个具有DNA结合域(DBD)、寡聚化结构域(OD)、核定位信号(NLS)、核输出信号(NES)和AHA型激活结构域。OsHsf条目LOC_Os06g226100具有低聚结构域，但缺乏上述其他结构域。此外，在数据库中没有为该条目记录的EST或全长cDNA。表达谱分析表明，22个OsHsf基因受高温诱导。低温胁迫和氧化胁迫分别诱导了10个和14个OsHsf基因的表达。所有由氧化应激诱导的OsHsf基因也在高温下被诱导。另一方面，6个OsHsf基因和1个OsHsf基因的诱导分别是高温和低温胁迫所专有的。7个OsHsf基因在三种胁迫下均表现出诱导表达。而在电子启动子分析中，OsHsf基因含有对应于不同非生物胁迫的上游调控元件，而这些元件的电子表达谱与其相应的转录表达模式之间缺乏相关性。除了应激诱导，EST数据库表明，各种OsHsf基因在不同的组织类型中都受到发育调节。(C)2009年爱思唯尔·马森公司。版权所有。
Binding of heat shock factors (HSFs) with heat shock element sequence is critical for the transcriptional induction of heat shock genes. Rice genome sequence shows 26 OsHsf genes out of which 25 possess various important domains noted in HSFs i.e. DNA binding domain (DBD), oligomerization domain (OD), nuclear localization signal (NLS), nuclear export signal (NES) and AHA type activation domain. OsHsf entry LOC_Os06g226100 has the oligomerization domain but lacks the above other domains. Also, there are no ESTs or full-length cDNA noted for this entry in database. Expression profiling showed that 22 OsHsf genes are induced by high temperature. Induction of 10 and 14 OsHsf genes was also noted against low temperature stress and oxidative stress, respectively. All OsHsf genes induced by oxidative stress were also induced by high temperature. On the other hand, induction of 6 and 1 OsHsf genes was noted to be exclusive to high and low temperature stresses, respectively. Seven OsHsf genes showed induced expression in response to all the three stresses examined. While in silico promoter analysis showed that OsHsf genes contain upstream regulatory elements corresponding to different abiotic stresses, there was lack of correlation noted between the in silico profiling of the elements and their corresponding transcript expression patterns. Apart from stress inducibility, EST database suggests that various OsHsf genes are developmentally regulated in diverse tissue types. (C) 2009 Elsevier Masson SAS. All rights reserved.