RNAseq reveals weed-induced PIF3-like as a candidate target to manipulate weed stress response in soybean
RNAseq reveals weed-induced PIF3-like as a candidate target to manipulate weed stress response in soybean
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DOI：
10.1111/nph.13351
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发表时间：
2015-07-01
期刊：
影响因子：
9.4
通讯作者：
Clay, Sharon A.
中科院分区：
文献类型：
--
作者：
Horvath, David P.;Hansen, Stephanie A.;Clay, Sharon A.
杂草通过不完全确定的机制降低大豆（Glycine max）的产量。在四个不同生长季节的田间条件下评估了杂草对大豆转录组的影响。 RNASeq 数据是从有或没有杂草生长的大豆的六个生物样本中收集的。杂草种类和维持无杂草控制的方法因年份而异，以减轻处理效果，并允许检测一般大豆杂草反应。大豆植株没有明显的营养或水分胁迫。我们在杂草丛生的地块中发现了 55 个持续下调的基因。许多下调的基因是热休克基因。十四个基因持续上调。上调的基因中包括多种转录因子，包括 PHYTOCHROME INTERACTING FACTOR 3 样基因 (PIF3)。基因集富集分析表明杂草胁迫期间氧化应激和茉莉酸信号反应增加的作用。这种杂草诱导的 PIF3 基因与拟南芥中参与避荫反应的基因的关系提供了证据，表明该基因可能在大豆对杂草的反应中很重要。这些结果表明杂草诱导的 PIF3 基因将成为操纵大豆杂草耐受性的靶标。
Weeds reduce yield in soybeans (Glycine max) through incompletely defined mechanisms. The effects of weeds on the soybean transcriptome were evaluated in field conditions during four separate growing seasons. RNASeq data were collected from six biological samples of soybeans growing with or without weeds. Weed species and the methods to maintain weed-free controls varied between years to mitigate treatment effects, and to allow detection of general soybean weed responses. Soybean plants were not visibly nutrient- or water-stressed. We identified 55 consistently downregulated genes in weedy plots. Many of the downregulated genes were heat shock genes. Fourteen genes were consistently upregulated. Several transcription factors including a PHYTOCHROME INTERACTING FACTOR 3-like gene (PIF3) were included among the upregulated genes. Gene set enrichment analysis indicated roles for increased oxidative stress and jasmonic acid signaling responses during weed stress. The relationship of this weed-induced PIF3 gene to genes involved in shade avoidance responses in Arabidopsis provide evidence that this gene may be important in the response of soybean to weeds. These results suggest that the weed-induced PIF3 gene will be a target for manipulating weed tolerance in soybean.