Extracellular silica nanocoat formed by layer-by-layer (LBL) self-assembly confers aluminum resistance in root border cells of pea (Pisum sativum)
Extracellular silica nanocoat formed by layer-by-layer (LBL) self-assembly confers aluminum resistance in root border cells of pea (Pisum sativum)
复制标题
通过层层（LBL）自组装形成的细胞外二氧化硅纳米涂层赋予豌豆（Pisum sativum）根缘细胞抗铝性
DOI：
10.1186/s12951-019-0486-y
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发表时间：
2019-04-16
影响因子：
10.2
通讯作者：
Yu, Min
中科院分区：
文献类型：
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作者：
Feng, Yingming;Li, Xuewen;Yu, Min
土壤酸度（及其铝毒性）是限制全球作物生产和威胁全球粮食安全的主要因素。静电层层自组装（LBL）提供了一种方便和通用的方法来形成细胞外二氧化硅纳米涂层，其具有保护细胞免受物理应力或有毒物质损伤的能力。在这项工作中，我们已经测试了一个假设，即由LBL自组装形成的细胞外二氧化硅nanocoat将保护根边缘细胞（RBC），并提高他们的抵抗Al toxicity.ResultsScanning电子显微镜（SEM）和X射线光电子能谱（XPS）被用来比较与那些暴露于Al无涂层的纳米壳的RBC表面的属性。激光扫描共聚焦显微镜检测铝的积累、活性氧（ROS）水平和线粒体活性。我们发现，红细胞表面上的二氧化硅纳米颗粒的晶体状层的功能作为一个胞外铝防涂层，通过固定铝在质外体和防止其积累在胞质溶胶中。红细胞上的二氧化硅纳米壳对维持质膜和线粒体膜的完整性，防止ROS爆发，并确保更高的线粒体活性和细胞活力在Al toxicity.ConclusionsThe研究提供的证据表明，二氧化硅纳米壳赋予红细胞铝电阻通过抑制铝在二氧化硅涂层，这表明该方法可用于防止铝毒对农作物生产造成的数十亿美元损失的有效工具。
BackgroundSoil acidity (and associated Al toxicity) is a major factor limiting crop production worldwide and threatening global food security. Electrostatic layer-by-layer (LBL) self-assembly provides a convenient and versatile method to form an extracellular silica nanocoat, which possess the ability to protect cell from the damage of physical stress or toxic substances. In this work, we have tested a hypothesis that extracellular silica nanocoat formed by LBL self-assembly will protect root border cells (RBCs) and enhance their resistance to Al toxicity.ResultsScanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) were used to compare the properties of RBCs surface coated with nanoshells with those that were exposed to Al without coating. The accumulation of Al, reactive oxygen species (ROS) levels, and the activity of mitochondria were detected by a laser-scanning confocal microscopy. We found that a crystal-like layer of silica nanoparticles on the surface of RBCs functions as an extracellular Al-proof coat by immobilizing Al in the apoplast and preventing its accumulation in the cytosol. The silica nanoshells on the RBCs had a positive impact on maintaining the integrity of the plasma and mitochondrial membranes, preventing ROS burst and ensuring higher mitochondria activity and cell viability under Al toxicity.ConclusionsThe study provides evidence that silica nanoshells confers RBCs Al resistance by restraining of Al in the silica-coat, suggesting that this method can be used an efficient tool to prevent multibillion-dollar losses caused by Al toxicity to agricultural crop production.