Three different neutrophil subsets exhibited in mice with different susceptibilities to infection by methicillin-resistant Staphylococcus aureus
Three different neutrophil subsets exhibited in mice with different susceptibilities to infection by methicillin-resistant Staphylococcus aureus
复制标题
DOI：
10.1016/j.immuni.2004.07.006
复制
发表时间：
2004-08-01
期刊：
影响因子：
32.4
通讯作者：
Suzuki, F
中科院分区：
文献类型：
--
作者：
Tsuda, Y;Takahashi, H;Suzuki, F
中性粒细胞（PMN）被描述为宿主抗菌先天免疫中的关键效应细胞。然而，小鼠 PMN 的分类仍不清楚。在这里，我们发现除了正常的中性粒细胞（PMN-N）之外，至少还有两个不同的中性粒细胞亚群（PMN-I和PMN-II），其区别如下：（1）细胞因子和趋化因子产生（PMN-I，IL-12/CCL3；PMN-II，IL-10/CCL2；PMN-N，无细胞因子/趋化因子产生），（2）巨噬细胞激活 （PMN-I，经典激活的巨噬细胞；PMN-II，替代激活的巨噬细胞；PMN-N，对巨噬细胞激活没有影响），（3）Toll 样受体（TLR）表达（PMN-I，TLR2/TLR4/TLR5/TLR8；PMN-II，TLR2/TLR4/TLR7/TLR9；PMN-N， TLR2/TLR4/TLR9)，和(4)表面抗原表达(PMN-I、CD49d(+)CD11b(-)；PMN-II、CD49d(-)CD11b(+)；PMN-N、CD49d(-)CD11b(-))。 PMN-I是从MRSA（耐甲氧西林金黄色葡萄球菌）耐药宿主获得的，而MRSA敏感宿主是PMN-II的来源。 PMN-N 是从幼鼠身上获得的。抗 MRSA 先天免疫可能会受到这些生化和物理上不同的 PMN 的不同影响。 PMN-N 可以响应宿主环境而转化为 PMN-I 或 PMN-II。
Neutrophils (PMN) have been described as critical effector cells in the host's antibacterial innate immunities. However, the classification of murine PMNs remains unclear. Here, we show that in addition to normal PMN (PMN-N), there are at least two distinct subsets of PMNs (PMN-I and PMN-II) distinguished as follows: (1) cytokine and chemokine production (PMN-I, IL-12/CCL3; PMN-II, IL-10/CCL2; PMN-N, no cytokine/chemokine production), (2) macrophage activation (PMN-I, classically activated macrophages; PMN-II, alternatively activated macrophages; PMN-N, no effect on macrophage activation), (3) Toll-like receptor (TLR) expression (PMN-I, TLR2/TLR4/TLR5/ TLR8; PMN-II, TLR2/TLR4/TLR7/TLR9; PMN-N, TLR2/ TLR4/TLR9), and (4) surface antigen expression (PMN-I, CD49d(+)CD11b(-); PMN-II, CD49d(-)CD11b(+); PMN-N, CD49d(-)CD11b(-)). PMN-I was obtained from MRSA (methicillin-resistant Staphylococcus aureus)-resistant hosts, while MRSA-sensitive hosts were a source of PMN-II. PMN-N was obtained from naive mice. Anti-MRSA innate immunities might be influenced differently by these biochemically and physically distinguished PMNs. PMN-N may convert to PMN-I or PMN-II in response to host circumstance.