Orally administered LPS enhances head kidney macrophage activation with down-regulation of IL-6 in common carp (Cyprinus carpio)
Orally administered LPS enhances head kidney macrophage activation with down-regulation of IL-6 in common carp (Cyprinus carpio)
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DOI：
10.1016/j.fsi.2013.03.372
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发表时间：
2013-06-01
影响因子：
4.7
通讯作者：
Inagawa, Hiroyuki
中科院分区：
文献类型：
--
作者：
Kadowaki, Takeshi;Yasui, Yasumasa;Inagawa, Hiroyuki
免疫刺激剂代表了一种有前途的水产养殖工具，用于增强养殖鱼类的疾病和应激抗性。此外，免疫增强剂的作用时间和剂量也是养殖户关注的问题。本研究以鲤鱼为试验对象，研究了脂多糖（LPS）（5、10、20 μ g/kg/d）经口染毒鲤鱼30、60 d后的免疫指标变化。脂多糖能显著增强鲤鱼头肾巨噬细胞的吞噬和杀菌活性，并能增强血清溶菌酶活性。经口给予LPS增加了头肾中白细胞介素（IL）-1 β和TNF-α mRNA的表达，但降低了IL-6 mRNA的表达。虽然在高剂量（>15 mg/kg）口服给药后在血清和肝脏中检测到LPS，但在低剂量（>15 mg/kg）口服给药后通过给予LPS特异性ELISA未检测到LPS。
Immunostimulants represent a promising aquaculture tool for enhancing disease and stress resistance in cultured fish. Moreover, the term and dose for acting immunostimulants is an important thing for fish farmer. This study investigated the immune parameters of common carp after oral administration of LPS (5, 10, 20 mu g/kg/days) for 30 and 60 days, which is considered to be the proper time period for acting in aquaculture. Phagocytic and bactericidal activities of head kidney macrophages and serum lysozyme activities were significantly enhanced in LPS-fed carp. Orally administered LPS augmented the expression of interleukin (IL)-1 beta and TNF-alpha mRNAs but reduced the expression of IL-6 mRNA in head kidney. Although LPS was detected in the serum and liver after a high-dose (>15 mg/kg) oral administration, it was not detected by administered LPS-specific ELISA after a low-dose (