A second NAD(+)-dependent DNA ligase (LigB) in Escherichia coli.
A second NAD(+)-dependent DNA ligase (LigB) in Escherichia coli.
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DOI：
10.1093/nar/29.24.4930
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发表时间：
2001-12
影响因子：
14.9
通讯作者：
V. Sriskanda;S. Shuman
中科院分区：
文献类型：
--
作者：
V. Sriskanda;S. Shuman
Escherichia coli DNA ligase (LigA) is the prototype of the NAD(+)-dependent class of DNA ligases found in all bacteria. Here we report the characterization of E.coli LigB, a second NAD(+)-dependent DNA ligase identified by virtue of its sequence similarity to LigA. LigB differs from LigA in that it lacks the BRCA1 C-terminus domain (BRCT) and two of the four Zn-binding cysteines that are present in LigA and all other bacterial NAD(+) ligases. We found that recombinant LigB catalyzed strand joining on a singly-nicked DNA in the presence of a divalent cation and NAD(+), and that LigB reacted with NAD(+) to form a covalent ligase-adenylate intermediate. Alanine substitution for the motif I lysine ((126)KxDG) abolished nick joining and ligase-adenylate formation by LigB, thus confirming that the ligase and adenylyltransferase activities are intrinsic to the LigB protein.
