Aggregation and conformational change of mushroom (Agaricus bisporus) polyphenoloxidase subjected to thermal treatment
Aggregation and conformational change of mushroom (Agaricus bisporus) polyphenoloxidase subjected to thermal treatment
复制标题
热处理后蘑菇（双孢蘑菇）多酚氧化酶的聚集和构象变化
DOI：
10.1016/j.foodchem.2016.07.041
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发表时间：
2017-01-01
期刊：
影响因子：
8.8
通讯作者：
Chen, Jun
中科院分区：
文献类型：
--
作者：
Zhou, Lei;Liu, Wei;Chen, Jun
研究了热处理对蘑菇多酚氧化酶（PPO）活性、构象和微观结构的影响。高温短时间或温和长时间处理均可使PPO失活。圆二色谱和荧光光谱表明，加热过程引起的二级结构的重排和三级结构的破坏。荧光光谱的红移与PPO的失活率呈正相关。在45 ℃、55 ℃和65 ℃下处理不同时间得到的相对活性相同的PPO样品，其构象和分子微观结构存在显著差异。总之，PPO分子在温和的温度下变形，而较高的温度诱导形成大的聚集体。相对活性相同的PPO可能以不同的形式存在。(C)2016爱思唯尔有限公司版权所有
This study investigated changes in the activity, conformation and microstructure of mushroom polyphenoloxidase (PPO) subjected to thermal treatment. The inactivation of PPO can be achieved by high temperature-short time or mild temperature-long time treatment. Circular dichroism and fluorescence spectra suggested that heating process induced the rearrangement of secondary structure and the disruption of tertiary structure. Red shifts of fluorescence spectra showed positive correlations with the inactivation rate of PPO. There were significant differences in the conformation and molecular microstructure among PPO samples with the same relative activity, which were obtained by treating PPO at 45, 55 and 65 degrees C for different times. In summary, PPO molecules were deformed at mild temperature, while higher temperature induced the formation of large aggregates. PPO with the same relative activity might exist in different forms. (C) 2016 Elsevier Ltd. All rights reserved.