Noninvasive Determination of Depth in Transmission Raman Spectroscopy in Turbid Media Based on Sample Differential Transmittance
Noninvasive Determination of Depth in Transmission Raman Spectroscopy in Turbid Media Based on Sample Differential Transmittance
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DOI：
10.1021/acs.analchem.7b01469
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发表时间：
2017-09-19
影响因子：
7.4
通讯作者：
Matousek, Pavel
中科院分区：
文献类型：
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作者：
Gardner, Benjamin;Stone, Nicholas;Matousek, Pavel
在这里，我们提出了一种简单的非侵入性方法，利用透射拉曼光谱来确定埋藏物体的深度。根据理论，从适当分离的谱峰产生的光子将在它们传播的介质中受到不同的光学性质的影响。这些差异可以影响作为传输路径长度的函数的拉曼峰的相对强度，从而信号产生的深度被固有地编码在光谱中。在一项概念验证研究中，仅通过外部校准，就可以完全根据主要吸收溶液印度墨水(0.1mU/mL；RMSE0.42 mm)和散射液(RMSE0.50 mm)中两个峰的相对强度来准确预测聚四氟乙烯(PTFE)层的深度。这种简单的方法首次提供了使用简单的透射率测量几何图形来非侵入性地识别埋藏物体的深度的可能性，例如乳房钙化。
Here we propose a simple noninvasive approach to determine the depth of a buried object using transmission Raman spectroscopy. In accordance with theory, the photons arising from spectral peaks that are suitably separated will be subjected to different optical properties in the media through which they travel. These differences can impact the relative intensities of Raman peaks as a function of the transmission path length, thereby the depth of signal generation is inherently encoded in the spectra. In a proof-of-concept study, through only external calibrations, it was possible to accurately predict the depth of Polytetrafluoroethylene (PTFE) layer purely on the basis of relative intensity of two peaks in a predominantly absorbing solution Indian ink (0.1 mu L/mL; RMSE 0.42 mm) and a scattering solution (RMSE 0.50 mm). This simple approach offers the possibility to noninvasively identify the depth of a buried object, such as breast calcifications, using simple transmission measurement geometries for the first time.