OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation.
OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation.
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OCT4B1 调节人牙髓细胞炎症的细胞应激反应
DOI：
10.1155/2017/2756891
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发表时间：
2017
影响因子：
--
通讯作者：
Wei X
中科院分区：
文献类型：
--
作者：
Liu L;Huang R;Yang R;Wei X
导论.感染和细胞凋亡是牙齿组织炎症的联合触发因素。八聚体结合转录因子4-B1（octamer-binding transcription factor 4-B1，OCT 4 B1）是OCT 4家族的一个新的剪接变异体，具有抗细胞凋亡的功能。然而，其在牙髓炎中的具体作用仍然未知。方法.为了研究OCT 4 B1对牙髓细胞（DPC）炎症的影响，采用原位杂交、实时荧光定量PCR和荧光原位杂交技术检测了OCT 4 B1在炎症牙髓组织和DPC中的表达。建立OCT 4 B1过表达的DPC模型，免疫印迹和免疫荧光染色证实，然后用脂多糖（LPS）刺激。Hoechst/PI染色和流式细胞仪检测细胞凋亡率。CCK-8法测定细胞存活率。结果原位杂交、实时荧光定量PCR和FISH检测结果显示OCT 4 B1在炎症牙髓组织和LPS刺激后的牙本质前体细胞中广泛表达。Western blot和免疫荧光染色显示OCT 4 B1转染后OCT 4 B1和OCT 4 B的表达增加。Hoechst/PI染色和流式细胞仪检测结果显示，转染后红/蓝荧光减少，细胞凋亡率降低（3.45%）。CCK 8证实pCDH-OCT 4 B1-flag细胞的存活率增加。结论. OCT 4 B1在DPC的炎症和凋亡中起重要作用。OCT 4 B可能与OCT 4 B1协同作用以减少细胞凋亡。
Introduction. Infection and apoptosis are combined triggers for inflammation in dental tissues. Octamer-binding transcription factor 4-B1 (OCT4B1), a novel spliced variant of OCT4 family, could respond to the cellular stress and possess antiapoptotic property. However, its specific role in dental pulpitis remains unknown. Methods. To investigate the effect of OCT4B1 on inflammation of dental pulp cells (DPCs), its expression in inflamed dental pulp tissues and DPCs was examined by in situ hybridization, real-time PCR, and FISH assay. OCT4B1 overexpressed DPCs model was established, confirmed by western blot and immunofluorescence staining, and then stimulated with Lipopolysaccharide (LPS). Apoptotic rate was determined by Hoechst/PI staining and FACS. Cell survival rate was calculated by CCK8 assay. Results. In situ hybridization, real-time PCR, and FISH assay revealed that OCT4B1 was extensively expressed in inflamed dental pulp tissues and DPCs with LPS stimulation. Western blot and immunofluorescence staining showed the expression of OCT4B1 and OCT4B increased after OCT4B1 transfection. Hoechst/PI staining and FACS demonstrated that less red/blue fluorescence was detected and apoptotic percentage decreased (3.45%) after transfection. CCK8 demonstrated that the survival rate of pCDH-OCT4B1-flag cells increased. Conclusions. OCT4B1 plays an essential role in inflammation and apoptosis of DPCs. OCT4B might operate synergistically with OCT4B1 to reduce apoptosis.