Disordering effect of ethanol at different depths in the bilayer of mouse brain membranes.
Disordering effect of ethanol at different depths in the bilayer of mouse brain membranes.
复制标题
乙醇在小鼠脑膜双层不同深度的紊乱作用。
DOI：
10.1111/j.1530-0277.1981.tb04898.x
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Chin,JH
中科院分区：
文献类型：
--
作者：
Goldstein,DB;Chin,JH
十四酸衍生物是研究生物膜的有用的自旋S标记，因为它们在双层中的位置是已知的。它们的长轴平行于磷脂的酰基链，其羧基位于磷脂的极性头基附近的表面。他们的电子顺磁共振(EPR)信号来自‘DOOXY’基团中的自由基，这些自由基可以沿着酰基链放置在不同的位置。因此，人们可以在不同的深度监测双层。用这些自旋标记确定的有序参数是与探针在膜中运动的幅度和频率有关的光谱测量。膜双层靠近表面比在疏水内部更有序，正如这些标记的硬脂酸衍生物^，‘-^以及核磁共振所显示的那样。随着深度的增加，链的柔韧性有一个梯度。双层中不同位置的这些物理特性的差异为研究乙醇的作用部位提供了机会。以5-羟基硬脂酸为自旋标记物，我们先前已经证明乙醇的加入会破坏体外生物膜？这
TEARIC acid derivatives are useful spin S labels for study of biomembranes because their position in the bilayer is known. Their long axes are oriented parallel to the acyl chains of phospholipids, and their carboxyl groups lie at the surface near the polar head groups of the phospholipids. Their electron paramagnetic resonance (EPR) signal comes from the free radical in the'doxyl'group, which can be placed at different positions along the acyl chain. Thus, one can monitor the bilayer at different depths. Order parameters determined with these spin labels are spectral measures related to the amplitude and frequency of motion of the probe in the membrane. Membrane bilayers are more ordered near the surface than in the hydrophobic interior, as can be shown with these labeled stearic acid derivative^,'-^ as well as with nuclear magnetic~ esonance.~ There is a gradient of chain flexibility with increasing depth. These differences in physical characteristics at different locations in a bilayer provide opportunity to examine the site of action of ethanol. Using 5-doxylstearic acid as spin label, we have previously shown that the addition of ethanol in vitro disorders biomembranes? This