Statin-induced bone morphogenetic protein (BMP) 2 expression during bone regeneration: an immunohistochemical study
Statin-induced bone morphogenetic protein (BMP) 2 expression during bone regeneration: an immunohistochemical study
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DOI：
10.1016/j.tripleo.2008.06.025
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Iseki, Shoichi
中科院分区：
文献类型：
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作者：
Alam, Shamiul;Ueki, Koichiro;Iseki, Shoichi
目标.本研究的目的是调查骨形态发生蛋白（BMP）2的表达后，植入他汀类药物和重组人BMP-2（rhBMP-2），并比较这些物质在兔鼻骨的骨再生能力，使用免疫组织学方法。将12只成年雄性日本白色家兔（n = 12;年龄12-16周龄，体重2.5-3.0 kg）分为3个实验组和1个对照组。在保留鼻膜的情况下，在鼻骨中总共创建了48个骨缺损，每只兔4个。在实验组中，1组植入溶于0.2 mL水中的10 mg他汀类药物和去端胶原海绵（ACS）;第二组植入5 μ g rhBMP-2和ACS;第三组仅植入ACS。对照组未植入任何材料。在手术后1、2和4周处死动物。取出已手术的部分并准备进行组织学评估。免疫组化法检测BMP-2的表达，荧光显微镜下观察BMP-2和Ki-67的双标。术后1、2、4周，他汀类药物/ACS组与rhBMP-2/ACS组之间无显著差异。与对照组相比，两个植入组中BMP-2染色阳性的细胞数量显著增加（P <0.0001）。BMP-2和Ki-67双标免疫组化染色阳性率在两组中相似。这项研究表明，他汀类/ACS植入物显示BMP-2表达和骨诱导活性，这是类似的rhBMP-2/ACS植入物。(Oral口腔外科医学口腔病理学口腔放射学Endod 2009; 107：22-29）
Objectives. The purpose of this study was to investigate bone morphogenetic protein (BMP) 2 expression after implantation of a statin and recombinant human BMP-2 (rhBMP-2) and to compare the bone regeneration capability of these substances in the rabbit nasal bone using immunohistologic methods.Study design. Twelve adult male Japanese white rabbits (n = 12; age 12-16 weeks, weight 2.5-3.0 kg) were divided into 3 experimental groups and 1 control group. A total of 48 bone defects, 4 per rabbit, were created in the nasal bone while preserving the nasal membrane. In the experimental groups, 1 group was implanted with 10 mg of a statin dissolved in 0.2 mL water with an atelocollagen sponge (ACS); the second group was implanted with 5 mu g rhBMP-2 with an ACS; and in the third group only the ACS was implanted. No material was implanted in the control group. Animals were killed at 1, 2, and 4 weeks after surgery. The parts that had been operated on were removed and prepared for histologic assessment. The expression of BMP-2 was evaluated using immunohistochemistry, and double-immunostaining for BMP-2 and Ki-67 was observed by fluorescent microscopy.Results. No significant differences were observed between the statin/ACS group and rhBMP-2/ACS group at 1, 2, and 4 weeks after surgery. The number of cells which stained positively for BMP-2 increased significantly in both of the implanted groups compared with the control group (P < .0001). The positive fluorescent double-immunostaining for BMP-2 and Ki-67 was similar in both implanted groups.Conclusion. This study suggests that statin/ACS implants show BMP-2 expression and osteoinductive activity that is similar to those of rhBMP-2/ACS implants. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009; 107: 22-29)