EFFECT OF ZINC ON PERITONEAL MACROPHAGES INVITRO
EFFECT OF ZINC ON PERITONEAL MACROPHAGES INVITRO
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DOI：
10.1128/iai.16.1.367-373.1977
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发表时间：
1977-01-01
影响因子：
3.1
通讯作者：
CAMPBELL, JB
中科院分区：
文献类型：
--
作者：
CHVAPIL, M;STANKOVA, L;CAMPBELL, JB
大鼠腹腔巨噬细胞培养在培养基中与10%自体血清显示50%的抑制O2消耗0.4 mM锌激活时，只有酵母。锌离子对静息巨噬细胞无影响。Zn ~（2+）对巨噬细胞增殖的抑制作用与酵母颗粒与巨噬细胞的比例有关，随着激活细胞数的增加，抑制作用逐渐减弱，显微镜和扫描电镜显示，在0.1和0.5mM Zn ~（2+）存在下，完整的巨噬细胞由多形变为圆形。当用无锌培养基代替孵育培养基时，细胞恢复并变得不规则形状。锌对细胞形态影响的可逆性与巨噬细胞对锌的摄取和释放有关。培养基中血清的存在干扰了巨噬细胞对锌的吸收。除了已经报道的锌对肥大细胞、血小板和粒细胞的影响外，锌离子显然还抑制巨噬细胞的某些功能。
Rat peritoneal macrophages incubated in a medium with 10% autologous serum showed 50% inhibition of O2 consumption by 0.4 mM Zn only when activated by yeast. Zn ions had no effect on resting macrophages. The inhibitory efficiency of Zn depanded on the ratio of yeast particles to macrophages; with more activated cells, the inhibition was less. The multiple-shaped intact macrophages changed into rounded cells in the presence of 0.1 and 0.5 mM Zn2+ as shown by photomicrography and scanning electron microscopy. When the incubation medium was replaced by Zn-free medium, cells recovered and became irregularly shaped. The reversibility of Zn effect on cell morphology corresponded with uptake and release of Zn by macrophages. The presence of serum in the medium interfered with the magnitude of Zn uptake by macrophages. Besides already reported effects of Zn on mast cells, platelets, and granulocytes, Zn ions apparently also inhibit some functions of macrophages.