Effects of DL-alpha-difluoromethylornithine on Leishmania donovani promastigotes.
Effects of DL-alpha-difluoromethylornithine on Leishmania donovani promastigotes.
复制标题
DL-α-二氟甲基鸟氨酸对杜氏利什曼原虫前鞭毛体的影响。
DOI：
10.1111/j.1550-7408.1986.tb05654.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Ullman,B
中科院分区：
文献类型：
--
作者：
Kaur,K;Emmett,K;McCann,PP;Sjoerdsma,A;Ullman,B
Alpha‐difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase, has been demonstrated to be an effective agent against a variety of parasitic protozoa but not againstLeishmaniaspp. In this report, we show that Leishmania donovani promastigotes in continuous culture are sensitive to the growth inhibitory and cytotoxic effects of DFMO. Incubation of the promastigotes with DFMO obliterates intracellular putrescine pools and depletes spermidine concentrations, which correlates with the onset of growth inhibition. The effects of DFMO on the growth and the intracellular polyamine pools can be reversed completely by the addition of 10 μM putrescine to the culture medium. These results suggest that the treatment of leishmaniasis may be amenable to chemotherapeutic manipulation by DFMO.
