Detection of invasive protein profile of Streptococcus pyogenes M1 isolates from pharyngitis patients
Detection of invasive protein profile of Streptococcus pyogenes M1 isolates from pharyngitis patients
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DOI：
10.1111/j.1600-0463.2009.02574.x
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发表时间：
2010-03-01
期刊：
影响因子：
2.8
通讯作者：
Ohta, Michio
中科院分区：
文献类型：
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作者：
Hasegawa, Tadao;Okamoto, Akira;Ohta, Michio
链球菌中毒性休克综合征（STSS）是日本和许多其他发达国家重新出现的传染病。流行病学研究表明，化脓性链球菌M1血清型是STSS最主要的致病菌株。最近对M1分离株的表征表明，covS突变是双组分调控系统之一，通过改变蛋白表达在STSS中起重要作用。我们分析了M1 S采用双向凝胶电泳（2-DE）和脉冲场凝胶电泳（PFGE）对1990年前后日本化脓性链球菌临床分离株进行了研究。PFGE图谱在1990年前后的分离物之间是不同的。1990年以后从STSS和咽炎患者分离的菌株之间存在显著差异。双组分调控系统的序列分析表明，covS突变不仅在STSS中检测到，而且在三个咽炎分离株，其中蛋白质从培养上清液显示侵袭型。通过2-DE分析，在咽炎分离株中检测到的突变的CovS对链球菌热原性外毒素B（SpeB）的产生具有受损的功能。这些结果表明，即使在咽部感染中也发生了导致CovS蛋白功能障碍的几种CovS突变。
Streptococcal toxic shock syndrome (STSS) is a re-emerging infectious disease in Japan and many other developed countries. Epidemiological studies have revealed that the M1 serotype of Streptococcus pyogenes is the most dominant causative isolate of STSS. Recent characterization of M1 isolates revealed that the mutation of covS, one of the two-component regulatory systems, plays an important role in STSS by altering protein expression. We analyzed the M1 S. pyogenes clinical isolates before or after 1990 in Japan, using two-dimensional gel electrophoresis (2-DE) and pulsed-field gel electrophoresis (PFGE). PFGE profiles were different between the isolates before and after 1990. Markedly different profiles among isolates after 1990 from STSS and pharyngitis patients were detected. Sequence analysis of two-component regulatory systems showed that covS mutations were detected not only in STSS but also in three pharyngitis isolates, in which proteins from the culture supernatant displayed the invasive type. The mutated CovS detected in the pharyngitis isolates had impaired function on the production of streptococcal pyrogenic exotoxin B (SpeB) analyzed by 2-DE. These results suggest that several covS mutations that lead to the malfunction of the CovS protein occurred even in pharyngeal infection.