Influence of biofilm formation on the optical properties of novel bioactive glass-containing composites.
Influence of biofilm formation on the optical properties of novel bioactive glass-containing composites.
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DOI：
10.1016/j.dental.2016.06.011
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发表时间：
2016-09
期刊：
影响因子：
--
通讯作者：
Ferracane JL
中科院分区：
文献类型：
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作者：
Hyun HK;Ferracane JL
生物活性玻璃（BAG）由于其抗菌作用和促进体液中磷灰石形成的潜力，已被建议作为牙科修复材料的可能添加剂。本研究的目的是研究细菌生物膜对具有不同初始表面粗糙度的新型含细菌生物膜复合材料的色度值和透光性的影响。通过用BAG（65摩尔% Si; 4% P; 31% Ca）、BAG-F（61% Si; 31% Ca; 4% P; 3% F; 1% B）或硅烷化二氧化硅替换15%硅烷化Sr玻璃来制备具有72重量%总填料负载的复合材料。将2 mm厚的光固化圆盘（n=10/组）分为4个不同的表面粗糙度亚组，这些亚组通过用600粒度SiC湿抛光产生，然后高达1200、2400或4000粒度SiC。在含有或不含有S的培养基中孵育之前和之后，测量CIE L*a*B*，并计算色差和透光参数（TP）。变形杆菌（UA 159）生物膜培养2周（无搅拌）。使用ANOVA/Tukey检验（α = 0.05）分析结果。与仅培养基相比，BAG和BAG-F复合材料的所有颜色差异显示细菌生物膜显著降低。老化前BAG和BAG-F复合材料的平均TP（SD）[10.0（2.8）和8.5（1.4）]高于对照复合材料[4.9（0.8）]，而TP随老化的变化大于有或无细菌的对照。具有最光滑表面的BAG-F复合材料与BAG复合材料相比在细菌生物膜下显示出更大的TP降低。当暴露于细菌生物膜时，含有生物活性玻璃添加剂的高度抛光的牙科复合材料可能会变得稍微粗糙并显示出降低的半透明性，但不会比不含BAG的对照复合材料变色更多。
Bioactive glass (BAG) has been suggested as a possible additive for dental restorative materials because of its antimicrobial effect and potential for promoting apatite formation in body fluids. The purpose of this study was to investigate the effects of bacterial biofilm on the change of colorimetric value and translucency of novel BAG-containing composites having different initial surface roughness. Composites with 72 wt% total filler load were prepared by replacing 15% of the silanized Sr glass with BAG (65 mole% Si; 4% P; 31% Ca), BAG-F (61% Si; 31% Ca; 4% P; 3% F; 1% B), or silanized silica. Light-cured discs of 2-mm thickness (n=10/group) were divided into 4 different surface roughness subgroups produced by wet polishing with 600 and then up to 1200, 2400, or 4000 grit SiC. CIE L*a*b* were measured and the color difference and translucency parameter (TP) were calculated before and after incubating in media with or without a S. mutans (UA 159) biofilm for 2 wks (no agitation). Results were analyzed using ANOVA/Tukey's test (α = 0.05). All the color differences for BAG and BAG-F composite showed significant decreases with bacterial biofilm compared to media-only. The mean TP (SD) of BAG and BAG-F composite before aging [10.0 (2.8) and 8.5 (1.4)] was higher than that of the control composite [4.9 (0.8)], while the change in TP with aging was greater compared to the control with or without bacteria. BAG-F composites with the smoothest surfaces showed a greater decrease in TP under bacterial biofilm compared to the BAG composite. Highly polished dental composites containing bioactive glass additives may become slightly rougher and show reduced translucency when exposed to bacterial biofilms, but do not discolor any more than control composites that do not contain the BAG.