Mass spectrometric analysis of the marine lipophilic biotoxins pectenotoxin-2 and okadaic acid by four different types of mass spectrometers
Mass spectrometric analysis of the marine lipophilic biotoxins pectenotoxin-2 and okadaic acid by four different types of mass spectrometers
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DOI：
10.1002/jms.1407
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发表时间：
2008-08-01
影响因子：
2.3
通讯作者：
de Boer, Jacob
中科院分区：
文献类型：
--
作者：
Gerssen, Arjen;Mulder, Patrick;de Boer, Jacob
研究了四种不同质谱仪 [三重四极杆 (TQ)、飞行时间 (ToF)、四极杆 ToF (Q-ToF) 和离子阱 (IT)] 检测海洋亲脂性毒素果胶毒素-2 (PTX2) 和冈田酸 (OA) 的性能。使用不同质谱分析仪获得的光谱数据用于提出正电喷雾模式下的 PTX2 和负电喷雾模式下的 OA 的裂解方案。 TQ 数据用于获得产物离子，而 ToF 和 Q-ToF-MS 分别产生前体离子和产物离子的精确质量数据。 IT 数据使用 MS 实验提供了对碎片途径的更好理解。就分析性能而言，所有四台质量分析仪均表现出良好的线性度 (R-2 > 0.97) 和重复性 (CV
The performances of four different mass spectrometers [triple-quadrupole (TQ), time-of-flight (ToF), quadrupole ToF (Q-ToF) and ion trap (IT)] for the detection of the marine lipophilic toxins pectenotoxin-2 (PTX2) and okadaic acid (OA) were investigated. The spectral data obtained with the different mass spectrometric analyzers were used to propose fragmentation schemes for PTX2 in the positive electrospray mode and for OA in the negative electrospray mode. TQ data were used to obtain product ions, while ToF and Q-ToF-MS produced accurate mass data of the precursor ion and product ions, respectively. IT data provided a better understanding of the fragmentation pathways using MS '' experiments. With respect to analytical performance, all four mass analyzers showed a good linearity (R-2 > 0.97) and repeatability (CV