Insulin, transforming growth factors, and substrates modulate growth of guinea pig pancreatic duct cells in vitro.
Insulin, transforming growth factors, and substrates modulate growth of guinea pig pancreatic duct cells in vitro.
复制标题
胰岛素、转化生长因子和底物在体外调节豚鼠胰管细胞的生长。
DOI：
10.1016/0016-5085(95)90405-0
复制
发表时间：
1995
期刊：
影响因子：
29.4
通讯作者：
Hootman,SR
中科院分区：
文献类型：
--
作者：
Bhattacharyya,E;Panchal,A;Wilkins,TJ;deOndarza,J;Hootman,SR
背景与目的人们对控制胰腺排泄管系统细胞更新的生理机制知之甚少。本研究调查了潜在调节物质对培养的豚鼠胰管上皮单层生长的影响。方法在存在和不存在不同物质的情况下，将胰管外植体在塑料和渗透性过滤器上培养3天。通过 5-溴-2'-脱氧尿苷掺入和形态测量程序测量这些外植体的上皮单层生长。结果表皮生长因子和胰岛素均增强单层生长，并共同产生相加效应。转化生长因子α增强生长，转化生长因子β抑制生长，而胰高血糖素、生长抑素、胰多肽、促胰液素、雨蛙素、铃蟾肽和地塞米松没有显着影响。与在组织培养塑料上生长的单层细胞相比，I 型胶原涂层过滤器上的单层细胞生长得到增强。外植体在涂有 IV 型胶原和纤连蛋白的滤膜上的细胞生长与在塑料上的相当，而在基质胶或层粘连蛋白涂敷的滤膜上的生长则减少。结论胰岛素、转化生长因子和底物成分在体外调节胰管上皮细胞的生长，表明它们是完整胰腺排泄管系统中细胞分裂的重要调节剂。
Background & AimsLittle is known of the physiological mechanisms that control cellular renewal in the pancreatic excretory duct system. This study investigated the effects of potential regulatory substances on the growth of cultured guinea pig pancreatic duct epithelial monolayers.MethodsPancreatic duct explants were cultured for 3 days on plastic and on permeable filters in the presence and absence of different substances. Growth of epithelial monolayers from these explants was measured by 5-bromo-2′-deoxyuridine incorporation and morphometric procedures.ResultsEpidermal growth factor and insulin both enhanced monolayer growth and together had an additive effect. Transforming growth factor α enhanced and transforming growth factor β inhibited growth, whereas glucagon, somatostatin, pancreatic polypeptide, secretin, cerulein, bombesin, and dexamethasone had no significant effects. Monolayer growth on type I collagen-coated filters was enhanced when compared with that of monolayers grown on tissue culture plastic. Cell growth from explants on filters coated with type IV collagen and fibronectin was comparable with that on plastic, whereas growth on Matrigel- or laminin-coated filters was reduced.ConclusionsInsulin, transforming growth factors, and substrate components modulate growth of pancreatic duct epithelial cells in vitro, suggesting that they are important regulators of cell division in the excretory duct system of the intact pancreas.