Vascular endothelial growth factor levels and induction of permeability in malignant pleural effusions.
Vascular endothelial growth factor levels and induction of permeability in malignant pleural effusions.
复制标题
DOI：
--
复制
发表时间：
1999-11
期刊：
影响因子：
--
通讯作者：
Brian K. Zebrowski;S. Yano;Wenbiao Liu;R. M. Shaheen;D. Hicklin;J. Putnam;L. Ellis
中科院分区：
文献类型：
--
作者：
Brian K. Zebrowski;S. Yano;Wenbiao Liu;R. M. Shaheen;D. Hicklin;J. Putnam;L. Ellis
血管内皮生长因子（VEGF）是血管生成和血管通透性的重要介质。我们假设恶性胸腔积液可能含有高水平的VEGF蛋白以及参与这些过程的其他细胞因子。收集了39例不同类型癌症患者经细胞学证实为恶性的胸腔积液，并通过免疫测定法测定了积液中VEGF、白细胞介素-8和血管生成素的水平。阴性对照是来自健康个体的非恶性腹水和血清样品。在继发于乳腺癌、间皮瘤和非小细胞肺癌的胸腔积液中以及合并所有恶性胸腔积液样本时，VEGF水平显著高于对照样本。与对照组相比，恶性胸腔积液中的白细胞介素-8和血管生成素水平均未升高。血管渗透性，这是通过使用迈尔斯测定在裸鼠中，增加成比例与VEGF水平的恶性胸腔积液;这种增加的渗透性诱导的注射重组VEGF或恶性积液减少预处理的小鼠与VEGF受体抗体。
Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis and vascular permeability. We hypothesized that malignant pleural effusions may contain high levels of VEGF protein as well as other cytokines implicated in these processes. Pleural effusions cytologically proven to be malignant were collected from 39 patients with various types of cancer, and VEGF, interleukin-8, and angiogenin levels in the effusions were determined by immunoassay. Negative controls were nonmalignant ascites and serum samples from healthy individuals. VEGF levels were significantly higher than those of control samples in pleural effusions secondary to breast, mesothelioma, and non-small cell lung cancer and when all malignant pleural effusion samples were pooled. Neither interleukin-8 nor angiogenin levels were elevated in malignant pleural effusions relative to the control samples. Vascular permeability, which was measured by using the Miles assay in nude mice, was increased proportionately with VEGF levels in the malignant pleural effusions; this increase in permeability induced by injection of recombinant VEGF or the malignant effusions was reduced by pretreating the mice with a VEGF receptor antibody.