Morphology Change from Nanotube to Vesicle and Monolayer/Bilayer Alteration by Amphiphilic Block Polypeptides Having Aromatic Groups at C Terminal
Morphology Change from Nanotube to Vesicle and Monolayer/Bilayer Alteration by Amphiphilic Block Polypeptides Having Aromatic Groups at C Terminal
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DOI：
10.1246/bcsj.20170044
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发表时间：
2017-03
影响因子：
4
通讯作者：
Cheol Joo Kim;Saki Kurauchi;Tsuguaki Uebayashi;Ai Fujisaki;S. Kimura
中科院分区：
文献类型：
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作者：
Cheol Joo Kim;Saki Kurauchi;Tsuguaki Uebayashi;Ai Fujisaki;S. Kimura
本文合成了以亮氨酸为基础的疏水螺旋嵌段的C端具有芳香基团的两亲性嵌段多肽，如聚（肌氨酸）-b-(l-亮氨酸- aib)6-乙基咔唑、聚（肌氨酸）-b-(d-亮氨酸- aib)6-萘酰亚胺和聚（肌氨酸）-b-(d-亮氨酸- aib)6-卟啉。结果表明，聚(sarcos)-b-(l-Leu-Aib)6的弯曲薄片与C端附着的芳香基团明显不同，其分子结构为扭曲薄片、鞍状薄片和胶束。在90℃下加热后，三种具有芳香基团的两亲性多肽生成囊泡，而聚（肌氨酸）-b-(l-Leu-Aib)6的纳米管则相反。通过原子力显微镜对这些囊泡的精确观察，揭示了聚（肌氨酸）-b-(l-Leu-Aib)6-乙基唑的单层结构和聚（肌氨酸）-b-(d-Leu-Aib)6-萘酰亚胺和聚（肌氨酸）-b-(d-Leu-Aib)6-卟啉的双层结构。
Various amphiphilic block polypeptides having aromatic groups at the C terminal of a Leu-based hydrophobic helical block, poly(sarcosine)-b-(l-Leu-Aib)6-ethylcarbazole, poly(sarcosine)-b-(d-Leu-Aib)6-naphthalimide, and poly(sarcosine)-b-(d-Leu-Aib)6-porphyrin, were synthesized and self-assembled in water. Molecular assemblies of distorted sheet, saddle-like sheet, and micelle were identified, which were different from the curved sheet of poly(sarcosine)-b-(l-Leu-Aib)6 without any aromatic groups, indicating that the attachment of the aromatic groups to the C terminal significantly influenced the morphology. Upon heating at 90 °C, vesicles were generated with the three amphiphilic polypeptides with aromatic groups as opposed to the nanotubes of poly(sarcosine)-b-(l-Leu-Aib)6. The precise observations of these vesicles by AFM revealed formation of monolayer for poly(sarcosine)-b-(l-Leu-Aib)6-ethylcarbazole and bilayers for poly(sarcosine)-b-(d-Leu-Aib)6-naphthalimide and poly(sarcosine)-b-(d-Leu-Aib)6-porph...