Epigenetic regulation of spurious transcription initiation in Arabidopsis
Epigenetic regulation of spurious transcription initiation in Arabidopsis
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DOI：
10.1038/s41467-020-16951-w
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发表时间：
2020-06
影响因子：
16.6
通讯作者：
Ngoc Tu Le;Yoshiko Harukawa;Saori Miura;Damian Boer;A. Kawabe;H. Saze
中科院分区：
文献类型：
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作者：
Ngoc Tu Le;Yoshiko Harukawa;Saori Miura;Damian Boer;A. Kawabe;H. Saze
在植物中，表观遗传调控对于沉默转座子和维持适当的基因表达至关重要。然而，它对全基因组转录起始景观的影响仍然难以捉摸。通过使用基因表达帽分析（CAGE）测序对转录起始位点（tss）进行全基因组分析，我们发现数千个tss在拟南芥的各种表观遗传突变体中被特异性激活，称为隐型tss。许多在以前的研究中没有被发现，其中高达65%是由转座子引起的。它们具有与常规tss相似的遗传特征，它们的激活与RNAPII机制的异位募集密切相关。隐性tss的激活显著改变了附近tss的转录，包括那些对发育和应激反应重要的基因。因此，我们的研究揭示了表观遗传调控通过抑制隐性tss的转录在植物中维持适当的基因功能中的作用。
In plants, epigenetic regulation is critical for silencing transposons and maintaining proper gene expression. However, its impact on the genome-wide transcription initiation landscape remains elusive. By conducting a genome-wide analysis of transcription start sites (TSSs) using cap analysis of gene expression (CAGE) sequencing, we show that thousands of TSSs are exclusively activated in various epigenetic mutants ofArabidopsis thalianaand referred to as cryptic TSSs. Many have not been identified in previous studies, of which up to 65% are contributed by transposons. They possess similar genetic features to regular TSSs and their activation is strongly associated with the ectopic recruitment of RNAPII machinery. The activation of cryptic TSSs significantly alters transcription of nearby TSSs, including those of genes important for development and stress responses. Our study, therefore, sheds light on the role of epigenetic regulation in maintaining proper gene functions in plants by suppressing transcription from cryptic TSSs.